Insulin release by glucagon and secretin: studies with secretin-glucagon hybrids
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Insulin release by glucagon and secretin : studies with secretin-glucagon hybrids. / Kofod, Hans; Andreu, D; Thams, P; Merrifield, R B; Hedeskov, C J; Hansen, B; Lernmark, A.
In: American Journal of Physiology (Consolidated), Vol. 254, No. 4 Pt 1, 04.1988, p. E454-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Insulin release by glucagon and secretin
T2 - studies with secretin-glucagon hybrids
AU - Kofod, Hans
AU - Andreu, D
AU - Thams, P
AU - Merrifield, R B
AU - Hedeskov, C J
AU - Hansen, B
AU - Lernmark, A
PY - 1988/4
Y1 - 1988/4
N2 - Secretin and glucagon potentiate glucose-induced insulin release. We have compared the effects of secretin and glucagon with that of four hybrid molecules of the two hormones on insulin release and formation of cyclic AMP (cAMP) in isolated mouse pancreatic islets. All six peptides potentiated the release of insulin at 10 mM D-glucose, and their effects were indistinguishable with respect to the dynamics of release, dose-response relationship, and glucose dependency. However, measurements of cAMP accumulation in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (10(-4) M) showed that the fold increase compared with glucose alone had the following ranking order: secretin = [Tyr10, Tyr13]-secretin 1.6 less than [Tyr10, Tyr13, Trp25]secretin 1.8 less than glucagon 1.9 less than [Asp3, Glu9, Arg12]glucagon 2.3 = [Asp3, Glu9]glucagon. These results suggest that despite similar potentiating effects of secretin and glucagon on glucose-induced insulin release, their modes of action may be different.
AB - Secretin and glucagon potentiate glucose-induced insulin release. We have compared the effects of secretin and glucagon with that of four hybrid molecules of the two hormones on insulin release and formation of cyclic AMP (cAMP) in isolated mouse pancreatic islets. All six peptides potentiated the release of insulin at 10 mM D-glucose, and their effects were indistinguishable with respect to the dynamics of release, dose-response relationship, and glucose dependency. However, measurements of cAMP accumulation in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (10(-4) M) showed that the fold increase compared with glucose alone had the following ranking order: secretin = [Tyr10, Tyr13]-secretin 1.6 less than [Tyr10, Tyr13, Trp25]secretin 1.8 less than glucagon 1.9 less than [Asp3, Glu9, Arg12]glucagon 2.3 = [Asp3, Glu9]glucagon. These results suggest that despite similar potentiating effects of secretin and glucagon on glucose-induced insulin release, their modes of action may be different.
KW - Amino Acid Sequence
KW - Animals
KW - Cyclic AMP
KW - Glucagon
KW - Glucose
KW - Insulin
KW - Islets of Langerhans
KW - Kinetics
KW - Male
KW - Mice
KW - Mice, Inbred Strains
KW - Secretin
M3 - Journal article
C2 - 2833112
VL - 254
SP - E454-8
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
SN - 0363-6143
IS - 4 Pt 1
ER -
ID: 45575183