Inhibitory effect of mycoplasma-released arginase. Activity in mixed-lymphocyte and tumour cell cultures.
Research output: Contribution to journal › Journal article › Research › peer-review
Non-fermenting mycoplasma species deplete culture media for arginine through arginase activity linked to their arginine deiminase pathway, resulting in proliferation arrest and cell death in mycoplasma-contaminated cell cultures. The presence of only 2-3 Mycoplasma (M.) arginini-contaminated T cells in a one-way allogeneic mixed-lymphocyte culture (MLC) significantly inhibits development of cytotoxic T-cell activity. Likewise, strong degrees of inhibition are observed after addition of nanogram doses of M. arginini extracts (MAE) to MLC or cell proliferation cultures. M. arginini-induced cell inhibition can be reversed by addition of excess arginine to the culture medium. Antisera raised against non-fermenting, but not against fermenting, mycoplasma species block the inhibitory effect of MAE. SDS-PAGE separation of MAE disclosed a broad band at 60 kDa which contained arginase activity when assayed in MLC and cell proliferation culture. SDS-PAGE followed by western blotting and reaction with antisera raised against non-fermenting mycoplasma species demonstrated a band at 43 kDa common for these micro-organisms.
Original language | English |
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Journal | Scandinavian Journal of Immunology |
Volume | 32 |
Issue number | 6 |
Pages (from-to) | 623-30 |
Number of pages | 7 |
ISSN | 0300-9475 |
Publication status | Published - 1990 |
Bibliographical note
Keywords: Animals; Antibodies, Bacterial; Arginase; Blotting, Western; Cell Division; Cell Line; Growth Inhibitors; Humans; Lymphocyte Culture Test, Mixed; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Molecular Weight; Mycoplasma; T-Lymphocytes; T-Lymphocytes, Cytotoxic; Tumor Cells, Cultured
ID: 8746708