Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man
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Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man. / Ørskov, Lotte; Bak, Jens Friis; Abildgaard, Ulrik; Schmitz, Ole; Andreasen, F; Richter, Erik A.; Skjærbæk, C; Møller, N.
In: Diabetologia, Vol. 39, No. 2, 1996, p. 226-234.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man
AU - Ørskov, Lotte
AU - Bak, Jens Friis
AU - Abildgaard, Ulrik
AU - Schmitz, Ole
AU - Andreasen, F
AU - Richter, Erik A.
AU - Skjærbæk, C
AU - Møller, N
PY - 1996
Y1 - 1996
N2 - The purpose of the present study was to evaluate the role of muscle glycogen synthase activity in the reduction of glucose uptake during hypoglycaemia. Six healthy young men were examined twice; during 120 min of hyperinsulinaemic (1.5 mU.kg-1. min-1) euglycaemia followed by: 1)240 min of graded hypoglycaemia (plasma glucose nadir 2.8 mmol/l) or 2) 240 min of euglycaemia. At 350-360 min a muscle biopsy was taken and indirect calorimetry was performed at 210-240 and 330-350 min. Hypoglycaemia was associated with markedly increased levels of adrenaline, growth hormone and glucagon and also with less hyperinsulinaemia. During hypoglycaemia the fractional velocity for glycogen synthase was markedly reduced; from 29.8 +/- 2.3 to 6.4 +/- 0.9%, p < 0.05. Total glucose disposal was decreased during hypoglycaemia (5.58 +/- 0.55 vs 11.01 +/- 0.75 mg.kg-1. min-1 (euglycaemia); p < 0.05); this was primarily due to a reduction of non-oxidative glucose disposal (2.43 +/- 0.41 vs 7.15 +/- 0.7 mg.kg-1 .min-1 (euglycaemia); p < 0.05), whereas oxidative glucose disposal was only suppressed to a minor degree. In conclusion hypoglycaemia virtually abolishes the effect of insulin on muscle glycogen synthase activity. This is in keeping with the finding of a marked reduction of non-oxidative glucose metabolism.
AB - The purpose of the present study was to evaluate the role of muscle glycogen synthase activity in the reduction of glucose uptake during hypoglycaemia. Six healthy young men were examined twice; during 120 min of hyperinsulinaemic (1.5 mU.kg-1. min-1) euglycaemia followed by: 1)240 min of graded hypoglycaemia (plasma glucose nadir 2.8 mmol/l) or 2) 240 min of euglycaemia. At 350-360 min a muscle biopsy was taken and indirect calorimetry was performed at 210-240 and 330-350 min. Hypoglycaemia was associated with markedly increased levels of adrenaline, growth hormone and glucagon and also with less hyperinsulinaemia. During hypoglycaemia the fractional velocity for glycogen synthase was markedly reduced; from 29.8 +/- 2.3 to 6.4 +/- 0.9%, p < 0.05. Total glucose disposal was decreased during hypoglycaemia (5.58 +/- 0.55 vs 11.01 +/- 0.75 mg.kg-1. min-1 (euglycaemia); p < 0.05); this was primarily due to a reduction of non-oxidative glucose disposal (2.43 +/- 0.41 vs 7.15 +/- 0.7 mg.kg-1 .min-1 (euglycaemia); p < 0.05), whereas oxidative glucose disposal was only suppressed to a minor degree. In conclusion hypoglycaemia virtually abolishes the effect of insulin on muscle glycogen synthase activity. This is in keeping with the finding of a marked reduction of non-oxidative glucose metabolism.
KW - Adult
KW - Biopsy
KW - Blood Glucose
KW - C-Peptide
KW - Calorimetry, Indirect
KW - Epinephrine
KW - Fatty Acids, Nonesterified
KW - Glucagon
KW - Glucose Clamp Technique
KW - Glycogen Synthase
KW - Glycolysis
KW - Growth Hormone
KW - Humans
KW - Hypoglycemia
KW - Infusions, Intravenous
KW - Insulin
KW - Kinetics
KW - Male
KW - Muscle, Skeletal
KW - Reference Values
KW - Time Factors
M3 - Journal article
C2 - 8635676
VL - 39
SP - 226
EP - 234
JO - Diabetologia
JF - Diabetologia
SN - 0012-186X
IS - 2
ER -
ID: 154750300