Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability
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Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability. / Nilsson, Martin; Chiang, Wen-Chi; Fazli, Mustafa; Gjermansen, Morten; Givskov, Michael; Tolker-Nielsen, Tim.
In: Environmental Microbiology, Vol. 13, No. 5, 2011, p. 1357-69.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability
AU - Nilsson, Martin
AU - Chiang, Wen-Chi
AU - Fazli, Mustafa
AU - Gjermansen, Morten
AU - Givskov, Michael
AU - Tolker-Nielsen, Tim
N1 - © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.
PY - 2011
Y1 - 2011
N2 - We report a study of the role of putative exopolysaccharide gene clusters in the formation and stability of Pseudomonas putida KT2440 biofilm. Two novel putative exopolysaccharide gene clusters, pea and peb, were identified, and evidence is provided that they encode products that stabilize P. putida KT2440 biofilm. The gene clusters alg and bcs, which code for proteins mediating alginate and cellulose biosynthesis, were found to play minor roles in P. putida KT2440 biofilm formation and stability under the conditions tested. A P. putida KT2440 derivative devoid of any identifiable exopolysaccharide genes was found to form biofilm with a structure similar to wild-type biofilm, but with a stability lower than that of wild-type biofilm. Based on our data, we suggest that the formation of structured P. putida KT2440 biofilm can occur in the absence of exopolysaccharides; however, exopolysaccharides play a role as structural stabilizers.
AB - We report a study of the role of putative exopolysaccharide gene clusters in the formation and stability of Pseudomonas putida KT2440 biofilm. Two novel putative exopolysaccharide gene clusters, pea and peb, were identified, and evidence is provided that they encode products that stabilize P. putida KT2440 biofilm. The gene clusters alg and bcs, which code for proteins mediating alginate and cellulose biosynthesis, were found to play minor roles in P. putida KT2440 biofilm formation and stability under the conditions tested. A P. putida KT2440 derivative devoid of any identifiable exopolysaccharide genes was found to form biofilm with a structure similar to wild-type biofilm, but with a stability lower than that of wild-type biofilm. Based on our data, we suggest that the formation of structured P. putida KT2440 biofilm can occur in the absence of exopolysaccharides; however, exopolysaccharides play a role as structural stabilizers.
KW - Alginates
KW - Biofilms
KW - Cellulose
KW - Gene Knockout Techniques
KW - Glucuronic Acid
KW - Hexuronic Acids
KW - Multigene Family
KW - Mutation
KW - Plasmids
KW - Polysaccharides, Bacterial
KW - Pseudomonas putida
U2 - 10.1111/j.1462-2920.2011.02447.x
DO - 10.1111/j.1462-2920.2011.02447.x
M3 - Journal article
C2 - 21507178
VL - 13
SP - 1357
EP - 1369
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2912
IS - 5
ER -
ID: 36061697