Increased susceptibility to diet-induced obesity in histamine-deficient mice
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Increased susceptibility to diet-induced obesity in histamine-deficient mice. / Jørgensen, Emilie A; Vogelsang, Thomas W; Knigge, Ulrich; Watanabe, Takeshi; Warberg, Jørgen; Kjaer, Andreas.
In: Neuroendocrinology, Vol. 83, No. 5-6, 2006, p. 289-94.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Increased susceptibility to diet-induced obesity in histamine-deficient mice
AU - Jørgensen, Emilie A
AU - Vogelsang, Thomas W
AU - Knigge, Ulrich
AU - Watanabe, Takeshi
AU - Warberg, Jørgen
AU - Kjaer, Andreas
N1 - Keywords: Adipose Tissue; Animals; Body Weight; Dietary Fats; Energy Intake; Histamine; Histidine Decarboxylase; Hypothalamus; Leptin; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Obesity; RNA, Messenger; Receptors, Cell Surface; Receptors, Leptin; Statistics, Nonparametric
PY - 2006
Y1 - 2006
N2 - BACKGROUND AND AIM: The neurotransmitter histamine is involved in the regulation of appetite and in the development of age-related obesity in mice. Furthermore, histamine is a mediator of the anorexigenic action of leptin. The aim of the present study was to investigate a possible role of histamine in the development of high-fat diet (HFD)-induced obesity. METHODS: Histamine-deficient histidine decarboxylase knock-out (HDC-KO) mice and C57BL/6J wild-type (WT) mice were given either a standard diet (STD) or HFD for 8 weeks. Body weight, 24-hour caloric intake, epididymal adipose tissue size, plasma leptin concentration and quantitative expression of leptin receptor (Ob-R) mRNA were measured. RESULTS: Both HDC-KO and WT mice fed an HFD for 8 weeks increased their body weight significantly more than STD-fed mice. A significant difference in body weight gain between HDC-KO mice fed an HFD or an STD was seen after 2 weeks, whereas a significant difference in body weight gain was first observed after 5 weeks in WT mice. After 8 weeks 24-hour caloric intake was significantly lower in HFD- than in STD-fed WT mice. In HDC-KO mice no difference in caloric intake was observed between HFD- and STD-fed mice. After 8 weeks epididymal adipose tissue size and plasma leptin concentration had increased significantly in HFD-fed WT and HDC-KO mice compared to their STD-fed controls. Epididymal adipose tissue size was higher in HDC-KO than WT mice, both in STD- and HFD-fed mice. A significant decrease in Ob-R mRNA in HFD-fed HDC-KO mice compared to STD-fed HDC-KO mice was observed, while no such difference was observed in WT mice. CONCLUSION: Based on our results, we conclude that histamine plays a role in the development of HFD-induced obesity.
AB - BACKGROUND AND AIM: The neurotransmitter histamine is involved in the regulation of appetite and in the development of age-related obesity in mice. Furthermore, histamine is a mediator of the anorexigenic action of leptin. The aim of the present study was to investigate a possible role of histamine in the development of high-fat diet (HFD)-induced obesity. METHODS: Histamine-deficient histidine decarboxylase knock-out (HDC-KO) mice and C57BL/6J wild-type (WT) mice were given either a standard diet (STD) or HFD for 8 weeks. Body weight, 24-hour caloric intake, epididymal adipose tissue size, plasma leptin concentration and quantitative expression of leptin receptor (Ob-R) mRNA were measured. RESULTS: Both HDC-KO and WT mice fed an HFD for 8 weeks increased their body weight significantly more than STD-fed mice. A significant difference in body weight gain between HDC-KO mice fed an HFD or an STD was seen after 2 weeks, whereas a significant difference in body weight gain was first observed after 5 weeks in WT mice. After 8 weeks 24-hour caloric intake was significantly lower in HFD- than in STD-fed WT mice. In HDC-KO mice no difference in caloric intake was observed between HFD- and STD-fed mice. After 8 weeks epididymal adipose tissue size and plasma leptin concentration had increased significantly in HFD-fed WT and HDC-KO mice compared to their STD-fed controls. Epididymal adipose tissue size was higher in HDC-KO than WT mice, both in STD- and HFD-fed mice. A significant decrease in Ob-R mRNA in HFD-fed HDC-KO mice compared to STD-fed HDC-KO mice was observed, while no such difference was observed in WT mice. CONCLUSION: Based on our results, we conclude that histamine plays a role in the development of HFD-induced obesity.
U2 - 10.1159/000095339
DO - 10.1159/000095339
M3 - Journal article
C2 - 16926531
VL - 83
SP - 289
EP - 294
JO - Neuroendocrinology
JF - Neuroendocrinology
SN - 0028-3835
IS - 5-6
ER -
ID: 12796797