Immunological changes in human immunodeficiency virus (HIV)-infected individuals during HIV-specific protease inhibitor treatment
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Immunological changes in human immunodeficiency virus (HIV)-infected individuals during HIV-specific protease inhibitor treatment. / Ullum, H; Katzenstein, T; Aladdin, H; Nielsen, C; Sondergaard, S R; Gerstoft, J; Skinhoj, P; Pedersen, Bente Klarlund.
In: Scandinavian Journal of Immunology, Vol. 49, No. 5, 05.1999, p. 539-47.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Immunological changes in human immunodeficiency virus (HIV)-infected individuals during HIV-specific protease inhibitor treatment
AU - Ullum, H
AU - Katzenstein, T
AU - Aladdin, H
AU - Nielsen, C
AU - Sondergaard, S R
AU - Gerstoft, J
AU - Skinhoj, P
AU - Pedersen, Bente Klarlund
PY - 1999/5
Y1 - 1999/5
N2 - The present study examines the influence of effective anti-retroviral treatment on immune function, evaluated by a broad array of immunological tests. We followed 12 individuals infected with human immunodeficiency virus (HIV) for 6 months after initiation of combination anti-retroviral treatment including a protease inhibitor. Unstimulated and pokeweed mitogen (PWM)-, interleukin (IL)-2- and phytohaemagglutinin (PHA)-stimulated lymphocyte proliferative responses increased during follow-up reaching average levels from 1.3-fold (PHA) to 3.7-fold (PWM) above baseline values. The total CD4+ lymphocyte count increased mainly due to increases in numbers of CD4+ CD28+ and CD4+ CD45RO+ cells, whereas increases in numbers of CD4+ CD45RA+ cells contributed little to the increase in CD4+ cell count. The total cytotoxic T-cell (CTL) killing of autologous B cells infected with HIV-encoding recombinant Vaccinia virus was increased after 3-6 months, whereas the specific HIV-directed CTL activity and the concentration and lytic activity of natural killer (NK) cells were unchanged during follow-up. These results demonstrate that the initiation of a treatment including an HIV protease inhibitor is followed by an increase in lymphocyte proliferation and lymphocyte-mediated cytotoxicity. However, unchanged levels of specific HIV CTL and NK cell activity warn us that not all measures of immune function may respond simultaneously to anti-retroviral treatment.
AB - The present study examines the influence of effective anti-retroviral treatment on immune function, evaluated by a broad array of immunological tests. We followed 12 individuals infected with human immunodeficiency virus (HIV) for 6 months after initiation of combination anti-retroviral treatment including a protease inhibitor. Unstimulated and pokeweed mitogen (PWM)-, interleukin (IL)-2- and phytohaemagglutinin (PHA)-stimulated lymphocyte proliferative responses increased during follow-up reaching average levels from 1.3-fold (PHA) to 3.7-fold (PWM) above baseline values. The total CD4+ lymphocyte count increased mainly due to increases in numbers of CD4+ CD28+ and CD4+ CD45RO+ cells, whereas increases in numbers of CD4+ CD45RA+ cells contributed little to the increase in CD4+ cell count. The total cytotoxic T-cell (CTL) killing of autologous B cells infected with HIV-encoding recombinant Vaccinia virus was increased after 3-6 months, whereas the specific HIV-directed CTL activity and the concentration and lytic activity of natural killer (NK) cells were unchanged during follow-up. These results demonstrate that the initiation of a treatment including an HIV protease inhibitor is followed by an increase in lymphocyte proliferation and lymphocyte-mediated cytotoxicity. However, unchanged levels of specific HIV CTL and NK cell activity warn us that not all measures of immune function may respond simultaneously to anti-retroviral treatment.
KW - Adult
KW - Aged
KW - Anti-HIV Agents
KW - Antigens, CD
KW - CD4 Lymphocyte Count
KW - CD8-Positive T-Lymphocytes
KW - Female
KW - Follow-Up Studies
KW - HIV Infections
KW - HIV Protease Inhibitors
KW - HIV-1
KW - Humans
KW - Male
KW - Middle Aged
KW - Prospective Studies
KW - Ritonavir
KW - Viral Load
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 10320648
VL - 49
SP - 539
EP - 547
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 5
ER -
ID: 180572190