IL-33 induces IL-9 production in human CD4+ T cells and basophils
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IL-33 induces IL-9 production in human CD4+ T cells and basophils. / Blom, Lars; Poulsen, Britta Cathrina; Jensen, Bettina M.; Hansen, Anker; Poulsen, Lars K.
In: P L o S One, Vol. 6, No. 7, e21695, 2011.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - IL-33 induces IL-9 production in human CD4+ T cells and basophils
AU - Blom, Lars
AU - Poulsen, Britta Cathrina
AU - Jensen, Bettina M.
AU - Hansen, Anker
AU - Poulsen, Lars K.
PY - 2011
Y1 - 2011
N2 - IL-33, an IL-1 family member and ligand for the IL-1 receptor-related protein ST2, has been associated with induction of Th2 cytokines such as IL-4, IL-5, and IL-13. Here, we report that IL-33 can initiate IL-9 protein secretion in vitro in human CD4+ T cells and basophils isolated from peripheral blood. TGF-β has been described as a critical factor for IL-9 induction in Th2 cells; however, we found that TGF-β also induces co-production of IL-9 in purified, naïve (>99%) CD4(+)CD45RA(+)CD45RO(-)CD25(-) T cells differentiated towards a Th1 profile. Subsequently, it was demonstrated that TGF-β is important, although not an absolute requirement, for IL-9 production in CD4+ T cells. IL-9 production by purified (>95%) human basophils, cultured for 24 h with IL-3 or IL-33, was found, with a strong synergy between the two, likely to be explained by the IL-3 upregulated ST2 expression. Collectively, these data indicate that barrier functioning cells are important for the regulation of IL-9 production by immune cells in inflamed tissue.
AB - IL-33, an IL-1 family member and ligand for the IL-1 receptor-related protein ST2, has been associated with induction of Th2 cytokines such as IL-4, IL-5, and IL-13. Here, we report that IL-33 can initiate IL-9 protein secretion in vitro in human CD4+ T cells and basophils isolated from peripheral blood. TGF-β has been described as a critical factor for IL-9 induction in Th2 cells; however, we found that TGF-β also induces co-production of IL-9 in purified, naïve (>99%) CD4(+)CD45RA(+)CD45RO(-)CD25(-) T cells differentiated towards a Th1 profile. Subsequently, it was demonstrated that TGF-β is important, although not an absolute requirement, for IL-9 production in CD4+ T cells. IL-9 production by purified (>95%) human basophils, cultured for 24 h with IL-3 or IL-33, was found, with a strong synergy between the two, likely to be explained by the IL-3 upregulated ST2 expression. Collectively, these data indicate that barrier functioning cells are important for the regulation of IL-9 production by immune cells in inflamed tissue.
KW - Basophils
KW - CD4-Positive T-Lymphocytes
KW - Cell Separation
KW - Gene Expression Regulation
KW - Humans
KW - Interleukin-4
KW - Interleukin-9
KW - Interleukins
KW - RNA, Messenger
KW - Receptors, Cell Surface
KW - Th1 Cells
KW - Th2 Cells
KW - Transforming Growth Factor beta
U2 - 10.1371/journal.pone.0021695
DO - 10.1371/journal.pone.0021695
M3 - Journal article
C2 - 21765905
VL - 6
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 7
M1 - e21695
ER -
ID: 33729868