Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae
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Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae. / Belmont, Martin; Cazzamali, Giuseppe; Williamson, Michael; Hauser, Frank; Grimmelikhuijzen, Cornelis J P.
In: Biochemical and Biophysical Research Communications, Vol. 344, No. 1, 26.05.2006, p. 160-5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae
AU - Belmont, Martin
AU - Cazzamali, Giuseppe
AU - Williamson, Michael
AU - Hauser, Frank
AU - Grimmelikhuijzen, Cornelis J P
PY - 2006/5/26
Y1 - 2006/5/26
N2 - The mosquito Anopheles gambiae is an important vector for malaria, which is one of the most serious human parasitic diseases in the world, causing up to 2.7 million deaths yearly. To contribute to our understanding of A. gambiae and to the transmission of malaria, we have now cloned four evolutionarily related G protein-coupled receptors (GPCRs) from this mosquito and expressed them in Chinese hamster ovary cells. After screening of a library of thirty-three insect or other invertebrate neuropeptides and eight biogenic amines, we could identify (de-orphanize) three of these GPCRs as: an adipokinetic hormone (AKH) receptor (EC(50) for A. gambiae AKH, 3x10(-9)M), a corazonin receptor (EC(50) for A. gambiae corazonin, 4x10(-9)M), and a crustacean cardioactive peptide (CCAP) receptor (EC(50) for A. gambiae CCAP, 1x10(-9)M). The fourth GPCR remained an orphan, although its close evolutionary relationship to the A. gambiae and other insect AKH receptors suggested that it is a receptor for an AKH-like peptide. This is the first published report on evolutionarily related AKH, corazonin, and CCAP receptors in mosquitoes.
AB - The mosquito Anopheles gambiae is an important vector for malaria, which is one of the most serious human parasitic diseases in the world, causing up to 2.7 million deaths yearly. To contribute to our understanding of A. gambiae and to the transmission of malaria, we have now cloned four evolutionarily related G protein-coupled receptors (GPCRs) from this mosquito and expressed them in Chinese hamster ovary cells. After screening of a library of thirty-three insect or other invertebrate neuropeptides and eight biogenic amines, we could identify (de-orphanize) three of these GPCRs as: an adipokinetic hormone (AKH) receptor (EC(50) for A. gambiae AKH, 3x10(-9)M), a corazonin receptor (EC(50) for A. gambiae corazonin, 4x10(-9)M), and a crustacean cardioactive peptide (CCAP) receptor (EC(50) for A. gambiae CCAP, 1x10(-9)M). The fourth GPCR remained an orphan, although its close evolutionary relationship to the A. gambiae and other insect AKH receptors suggested that it is a receptor for an AKH-like peptide. This is the first published report on evolutionarily related AKH, corazonin, and CCAP receptors in mosquitoes.
KW - Amino Acid Sequence
KW - Animals
KW - Anopheles gambiae
KW - CHO Cells
KW - Cloning, Molecular
KW - Cricetinae
KW - Cricetulus
KW - Evolution, Molecular
KW - Gene Order
KW - Insect Hormones
KW - Insect Proteins
KW - Insect Vectors
KW - Malaria
KW - Molecular Sequence Data
KW - Neuropeptides
KW - Oligopeptides
KW - Phylogeny
KW - Pyrrolidonecarboxylic Acid
KW - Receptors, G-Protein-Coupled
KW - Receptors, Neuropeptide
U2 - 10.1016/j.bbrc.2006.03.117
DO - 10.1016/j.bbrc.2006.03.117
M3 - Journal article
C2 - 16616003
VL - 344
SP - 160
EP - 165
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -
ID: 33513349