Identification of common mechanisms by which human and mouse cytomegalovirus seven-transmembrane receptor homologues contribute to in vivo phenotypes in a mouse model
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Identification of common mechanisms by which human and mouse cytomegalovirus seven-transmembrane receptor homologues contribute to in vivo phenotypes in a mouse model. / Farrell, Helen E; Abraham, Alexander M; Cardin, Rhonda D; Mølleskov-Jensen, Ann-Sofie; Rosenkilde, Mette Marie; Davis-Poynter, Nicholas.
In: Journal of Virology, Vol. 87, No. 7, 04.2013, p. 4112-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of common mechanisms by which human and mouse cytomegalovirus seven-transmembrane receptor homologues contribute to in vivo phenotypes in a mouse model
AU - Farrell, Helen E
AU - Abraham, Alexander M
AU - Cardin, Rhonda D
AU - Mølleskov-Jensen, Ann-Sofie
AU - Rosenkilde, Mette Marie
AU - Davis-Poynter, Nicholas
PY - 2013/4
Y1 - 2013/4
N2 - The mouse cytomegalovirus chemokine receptor homologue (CKR) M33 is required for salivary gland tropism and efficient reactivation from latency, phenotypes partially rescued by the human cytomegalovirus CKR US28. Herein, we demonstrate that complementation of salivary gland tropism is mediated predominantly by G protein-dependent signaling conserved with that of M33; in contrast, both G protein-dependent and -independent pathways contribute to the latency phenotypes. A novel M33-dependent replication phenotype in cultured bone marrow macrophages is also described.
AB - The mouse cytomegalovirus chemokine receptor homologue (CKR) M33 is required for salivary gland tropism and efficient reactivation from latency, phenotypes partially rescued by the human cytomegalovirus CKR US28. Herein, we demonstrate that complementation of salivary gland tropism is mediated predominantly by G protein-dependent signaling conserved with that of M33; in contrast, both G protein-dependent and -independent pathways contribute to the latency phenotypes. A novel M33-dependent replication phenotype in cultured bone marrow macrophages is also described.
KW - Analysis of Variance
KW - Animals
KW - COS Cells
KW - Cercopithecus aethiops
KW - Cytomegalovirus
KW - HEK293 Cells
KW - Humans
KW - Luciferases
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, Inbred C57BL
KW - Octoxynol
KW - Phenotype
KW - Receptors, Chemokine
KW - Receptors, G-Protein-Coupled
KW - Salivary Glands
KW - Signal Transduction
KW - Viral Proteins
KW - Viral Tropism
KW - Virus Activation
KW - Virus Latency
U2 - 10.1128/JVI.03406-12
DO - 10.1128/JVI.03406-12
M3 - Journal article
C2 - 23345521
VL - 87
SP - 4112
EP - 4117
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 7
ER -
ID: 48972105