Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli

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Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli. / Magnusson, Lisa U.; Gummesson, Bertil; Joksimović, Predrag; Farewell, Anne; Nyström, Thomas.

In: Journal of Bacteriology, Vol. 189, No. 14, 01.07.2007, p. 5193-5202.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Magnusson, LU, Gummesson, B, Joksimović, P, Farewell, A & Nyström, T 2007, 'Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli', Journal of Bacteriology, vol. 189, no. 14, pp. 5193-5202. https://doi.org/10.1128/JB.00330-07

APA

Magnusson, L. U., Gummesson, B., Joksimović, P., Farewell, A., & Nyström, T. (2007). Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli. Journal of Bacteriology, 189(14), 5193-5202. https://doi.org/10.1128/JB.00330-07

Vancouver

Magnusson LU, Gummesson B, Joksimović P, Farewell A, Nyström T. Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli. Journal of Bacteriology. 2007 Jul 1;189(14):5193-5202. https://doi.org/10.1128/JB.00330-07

Author

Magnusson, Lisa U. ; Gummesson, Bertil ; Joksimović, Predrag ; Farewell, Anne ; Nyström, Thomas. / Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 14. pp. 5193-5202.

Bibtex

@article{de6152f15a704355a19df11bedc587ae,
title = "Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli",
abstract = "The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effects on many of the observed phenotypes but that DksA and ppGpp also have independent and sometimes opposing roles in the cell. Specifically, we show that overexpression of DksA can compensate for the loss of ppGpp with respect to transcription of the promoters PuspA, PlivJ, and P rrnBP1 as well as amino acid auxotrophy, cell-cell aggregation, motility, filamentation, and stationary phase morphology, suggesting that DksA can function without ppGpp in regulating gene expression. In addition, ppGpp and DksA have opposing effects on adhesion. In the course of our analysis, we also discovered new features of the relaxed mutant, namely, defects in cell-cell aggregation and motility.",
author = "Magnusson, {Lisa U.} and Bertil Gummesson and Predrag Joksimovi{\'c} and Anne Farewell and Thomas Nystr{\"o}m",
year = "2007",
month = jul,
day = "1",
doi = "10.1128/JB.00330-07",
language = "English",
volume = "189",
pages = "5193--5202",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "14",

}

RIS

TY - JOUR

T1 - Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli

AU - Magnusson, Lisa U.

AU - Gummesson, Bertil

AU - Joksimović, Predrag

AU - Farewell, Anne

AU - Nyström, Thomas

PY - 2007/7/1

Y1 - 2007/7/1

N2 - The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effects on many of the observed phenotypes but that DksA and ppGpp also have independent and sometimes opposing roles in the cell. Specifically, we show that overexpression of DksA can compensate for the loss of ppGpp with respect to transcription of the promoters PuspA, PlivJ, and P rrnBP1 as well as amino acid auxotrophy, cell-cell aggregation, motility, filamentation, and stationary phase morphology, suggesting that DksA can function without ppGpp in regulating gene expression. In addition, ppGpp and DksA have opposing effects on adhesion. In the course of our analysis, we also discovered new features of the relaxed mutant, namely, defects in cell-cell aggregation and motility.

AB - The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effects on many of the observed phenotypes but that DksA and ppGpp also have independent and sometimes opposing roles in the cell. Specifically, we show that overexpression of DksA can compensate for the loss of ppGpp with respect to transcription of the promoters PuspA, PlivJ, and P rrnBP1 as well as amino acid auxotrophy, cell-cell aggregation, motility, filamentation, and stationary phase morphology, suggesting that DksA can function without ppGpp in regulating gene expression. In addition, ppGpp and DksA have opposing effects on adhesion. In the course of our analysis, we also discovered new features of the relaxed mutant, namely, defects in cell-cell aggregation and motility.

UR - http://www.scopus.com/inward/record.url?scp=34447540221&partnerID=8YFLogxK

U2 - 10.1128/JB.00330-07

DO - 10.1128/JB.00330-07

M3 - Journal article

C2 - 17496080

AN - SCOPUS:34447540221

VL - 189

SP - 5193

EP - 5202

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 14

ER -

ID: 203861022