Hypotonic elution, a new desorption principle in immunoadsorbent chromatography

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Hypotonic elution, a new desorption principle in immunoadsorbent chromatography. / Danielsen, Erik Michael; Sjöström, H; Norén, O.

In: Journal of Immunological Methods, Vol. 52, No. 2, 1982, p. 223-32.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Danielsen, EM, Sjöström, H & Norén, O 1982, 'Hypotonic elution, a new desorption principle in immunoadsorbent chromatography', Journal of Immunological Methods, vol. 52, no. 2, pp. 223-32.

APA

Danielsen, E. M., Sjöström, H., & Norén, O. (1982). Hypotonic elution, a new desorption principle in immunoadsorbent chromatography. Journal of Immunological Methods, 52(2), 223-32.

Vancouver

Danielsen EM, Sjöström H, Norén O. Hypotonic elution, a new desorption principle in immunoadsorbent chromatography. Journal of Immunological Methods. 1982;52(2):223-32.

Author

Danielsen, Erik Michael ; Sjöström, H ; Norén, O. / Hypotonic elution, a new desorption principle in immunoadsorbent chromatography. In: Journal of Immunological Methods. 1982 ; Vol. 52, No. 2. pp. 223-32.

Bibtex

@article{a34f80c06c7a11de8bc9000ea68e967b,
title = "Hypotonic elution, a new desorption principle in immunoadsorbent chromatography",
abstract = "A largely unrecognized immunoadsorbent desorption technique, hypotonic elution, has been successfully used in the immunoadsorbent purification of the microvillar enzymes aminopeptidase N (EC 3.4.11.2), dipeptidyl peptidase IV (EC 3.4.14.5), sucrase-isomaltase (EC 3.2.1.48-10), lactase-phlorizin hydrolase (EC 3.2.1.23-62) and maltase-glucoamylase (EC 3.2.1.20). This elution method proved capable of achieving an acceptable yield (30-70%) while at the same time preserving the purified enzymes in an enzymically active state. It hereby offers a solution to the problem in immunoadsorbent chromatography of finding an efficient means of elution which is not denaturing to neither the purified enzyme nor the immunoadsorbent column. Common properties of the microvillar enzymes with regard to amphiphilicity, glycosylation or subunit composition could hypothetically account for the similar elution properties of the enzymes but were considered unlikely on several grounds. Hypotonic elution in immunoadsorbent chromatography, therefore, may have a much broader range of applicability, and the method is recommended to be tried out by workers in other areas of protein chemistry.",
author = "Danielsen, {Erik Michael} and H Sj{\"o}str{\"o}m and O Nor{\'e}n",
note = "Keywords: Aminopeptidases; Animals; Antigens, CD13; Cell Membrane; Chromatography; Hypotonic Solutions; Immunosorbent Techniques; Microvilli; Octoxynol; Polyethylene Glycols; Rabbits; Sucrase-Isomaltase Complex; Swine; Tromethamine",
year = "1982",
language = "English",
volume = "52",
pages = "223--32",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Hypotonic elution, a new desorption principle in immunoadsorbent chromatography

AU - Danielsen, Erik Michael

AU - Sjöström, H

AU - Norén, O

N1 - Keywords: Aminopeptidases; Animals; Antigens, CD13; Cell Membrane; Chromatography; Hypotonic Solutions; Immunosorbent Techniques; Microvilli; Octoxynol; Polyethylene Glycols; Rabbits; Sucrase-Isomaltase Complex; Swine; Tromethamine

PY - 1982

Y1 - 1982

N2 - A largely unrecognized immunoadsorbent desorption technique, hypotonic elution, has been successfully used in the immunoadsorbent purification of the microvillar enzymes aminopeptidase N (EC 3.4.11.2), dipeptidyl peptidase IV (EC 3.4.14.5), sucrase-isomaltase (EC 3.2.1.48-10), lactase-phlorizin hydrolase (EC 3.2.1.23-62) and maltase-glucoamylase (EC 3.2.1.20). This elution method proved capable of achieving an acceptable yield (30-70%) while at the same time preserving the purified enzymes in an enzymically active state. It hereby offers a solution to the problem in immunoadsorbent chromatography of finding an efficient means of elution which is not denaturing to neither the purified enzyme nor the immunoadsorbent column. Common properties of the microvillar enzymes with regard to amphiphilicity, glycosylation or subunit composition could hypothetically account for the similar elution properties of the enzymes but were considered unlikely on several grounds. Hypotonic elution in immunoadsorbent chromatography, therefore, may have a much broader range of applicability, and the method is recommended to be tried out by workers in other areas of protein chemistry.

AB - A largely unrecognized immunoadsorbent desorption technique, hypotonic elution, has been successfully used in the immunoadsorbent purification of the microvillar enzymes aminopeptidase N (EC 3.4.11.2), dipeptidyl peptidase IV (EC 3.4.14.5), sucrase-isomaltase (EC 3.2.1.48-10), lactase-phlorizin hydrolase (EC 3.2.1.23-62) and maltase-glucoamylase (EC 3.2.1.20). This elution method proved capable of achieving an acceptable yield (30-70%) while at the same time preserving the purified enzymes in an enzymically active state. It hereby offers a solution to the problem in immunoadsorbent chromatography of finding an efficient means of elution which is not denaturing to neither the purified enzyme nor the immunoadsorbent column. Common properties of the microvillar enzymes with regard to amphiphilicity, glycosylation or subunit composition could hypothetically account for the similar elution properties of the enzymes but were considered unlikely on several grounds. Hypotonic elution in immunoadsorbent chromatography, therefore, may have a much broader range of applicability, and the method is recommended to be tried out by workers in other areas of protein chemistry.

M3 - Journal article

C2 - 6126506

VL - 52

SP - 223

EP - 232

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 2

ER -

ID: 13063635