Human p53(264-272) HLA-A2 binding peptide is an immunodominant epitope in DNA-immunized HLA-A2 transgenic mice.
Research output: Contribution to journal › Journal article › Research › peer-review
C57BL/10 mice transgenic for HLA-A2 were immunized with either a full-length DNA-construct of the tumor suppressor p53 or with a minigene encoding the p53-derived immunodominant peptide p53(264)LLGRNSFEV272 (L9V). Vaccination with the full-length p53 construct induced potent cytotoxic activity of splenocytes against L9V-pulsed target cells after in vivo re-stimulation. Vaccination with the L9V-encoding minigene likewise induced specific anti-L9V cytotoxicity in vitro. Subsequent experiments revealed that peptide-pulsed dendritic cells were the most efficient cell types for in vitro re-stimulation. In concordance with this, immunization with L9V-pulsed dendritic cells also induced a potent and specific anti-L9V cytotoxic response in vitro. These data show that HLA-A2/peptide-specific cytotoxic immunity can be generated in vivo against the same immunodominant epitope by immunizing either with full-length DNA or with a DNA minigene encoding the immunodominant peptide epitope.
Original language | English |
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Journal | Cancer Letters |
Volume | 137 |
Issue number | 2 |
Pages (from-to) | 183-91 |
Number of pages | 8 |
ISSN | 0304-3835 |
Publication status | Published - 1999 |
Bibliographical note
Keywords: Animals; COS Cells; Cytotoxicity Tests, Immunologic; Dendritic Cells; Gene Expression; HLA-A2 Antigen; Humans; Immunity, Cellular; Immunodominant Epitopes; Mice; Mice, Inbred C57BL; Mice, Transgenic; Peptide Fragments; T-Lymphocytes, Cytotoxic; Transfection; Tumor Suppressor Protein p53; Vaccines, DNA
ID: 8746407