High-throughput siRNA screening applied to the ubiquitin-proteasome system
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High-throughput siRNA screening applied to the ubiquitin-proteasome system. / Poulsen, Esben Guldahl; Nielsen, Sofie V.; Pietras, Elin J.; Johansen, Jens Vilstrup; Steinhauer, Cornelia; Hartmann-Petersen, Rasmus.
Proteostasis. ed. / Rune Matthiesen. Springer, 2016. p. 421-439 (Methods in Molecular Biology, Vol. 1449).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - High-throughput siRNA screening applied to the ubiquitin-proteasome system
AU - Poulsen, Esben Guldahl
AU - Nielsen, Sofie V.
AU - Pietras, Elin J.
AU - Johansen, Jens Vilstrup
AU - Steinhauer, Cornelia
AU - Hartmann-Petersen, Rasmus
PY - 2016
Y1 - 2016
N2 - The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function.
AB - The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function.
KW - Journal Article
U2 - 10.1007/978-1-4939-3756-1_28
DO - 10.1007/978-1-4939-3756-1_28
M3 - Book chapter
C2 - 27613054
SN - 978-1-4939-3754-7
T3 - Methods in Molecular Biology
SP - 421
EP - 439
BT - Proteostasis
A2 - Matthiesen, Rune
PB - Springer
ER -
ID: 179133797