TY - JOUR

T1 - HGF is released from buccal fibroblasts after smokeless tobacco stimulation

AU - Dabelsteen, S

AU - Christensen, S

AU - Gron, B

AU - Bardow, A

AU - Dabelsteen, Erik

PY - 2005

Y1 - 2005

N2 - To investigate the effect of smokeless tobacco (ST) on (1) HGF, KGF and GM-CSF expression by buccal fibroblasts and (2) on keratinocyte and fibroblast proliferation. Buccal fibroblasts were stimulated with different concentrations of ST extracts in a double dilution from 0.50% w/v to 0.03% w/v. Supernatant was collected after 24/48/72/96 h and assayed for HGF, KGF, and GM-CSF by ELISA. The amount of RNA was used as an indicator of fibroblast cell number. Buccal epithelial cell proliferation was determined by CyQUANT proliferation assay. The amount of HGF and KGF in the supernatant was dependent on exposure time and on concentration of the tobacco extract. High concentration increased production of HGF 4-fold. KGF production was doubled when high concentration of tobacco was used, low concentration did not stimulate cells. GM-CSF production was low in both stimulated and non-stimulated cells. Keratinocytes and fibroblasts showed no increase in proliferation after stimulation with increased concentrations of ST. The results suggest that HGF and KGF may play an important role as a paracrine growth factor in epithelial hyperplasia in ST lesions.

AB - To investigate the effect of smokeless tobacco (ST) on (1) HGF, KGF and GM-CSF expression by buccal fibroblasts and (2) on keratinocyte and fibroblast proliferation. Buccal fibroblasts were stimulated with different concentrations of ST extracts in a double dilution from 0.50% w/v to 0.03% w/v. Supernatant was collected after 24/48/72/96 h and assayed for HGF, KGF, and GM-CSF by ELISA. The amount of RNA was used as an indicator of fibroblast cell number. Buccal epithelial cell proliferation was determined by CyQUANT proliferation assay. The amount of HGF and KGF in the supernatant was dependent on exposure time and on concentration of the tobacco extract. High concentration increased production of HGF 4-fold. KGF production was doubled when high concentration of tobacco was used, low concentration did not stimulate cells. GM-CSF production was low in both stimulated and non-stimulated cells. Keratinocytes and fibroblasts showed no increase in proliferation after stimulation with increased concentrations of ST. The results suggest that HGF and KGF may play an important role as a paracrine growth factor in epithelial hyperplasia in ST lesions.

KW - Adult

KW - Cell Proliferation

KW - Cells, Cultured

KW - Cheek

KW - Dose-Response Relationship, Drug

KW - Enzyme-Linked Immunosorbent Assay

KW - Fibroblast Growth Factor 7

KW - Fibroblast Growth Factors

KW - Fibroblasts

KW - Granulocyte-Macrophage Colony-Stimulating Factor

KW - Hepatocyte Growth Factor

KW - Humans

KW - Keratinocytes

KW - Mouth Mucosa

KW - Tobacco, Smokeless

U2 - 10.1016/j.oraloncology.2004.12.011

DO - 10.1016/j.oraloncology.2004.12.011

M3 - Journal article

C2 - 15878756

VL - 41

SP - 509

EP - 514

JO - Oral Oncology Extra

JF - Oral Oncology Extra

SN - 1741-9409

IS - 5

ER -