Growth hormone is a growth factor for the differentiated pancreatic beta-cell
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The regulation of the growth of the pancreatic beta-cell is poorly understood. There are previous indications of a role of GH in the growth and insulin production of the pancreatic islets. In the present study we present evidence for a direct long-term effect of GH on proliferation and insulin biosynthesis of pancreatic beta-cells in monolayer culture. In culture medium RPMI 1640 supplemented with 2% normal human serum islets or dissociated islet cells from newborn rats maintained their insulin-producing capacity. When supplemented with 1-1000 ng/ml pituitary or recombinant human GH the islet cells attached, spread out, and proliferated into monolayers mainly consisting of insulin-containing cells. The number of beta-cells in S-phase was increased from 0.9-6.5% as determined by immunochemical staining of bromodeoxyuridine incorporated into insulin-positive cells. The increase in cell number was accompanied with a continuous increase in insulin release to the culture medium reaching a 10- 20-fold increase after 2-3 months with a half-maximal effect at about 10 ng/ml human GH. The biosynthesis of (pro)insulin was markedly increased with a normal rate of conversion of proinsulin to insulin. It is concluded that GH is a potent growth factor for the differentiated pancreatic beta-cell.
Original language | English |
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Journal | Molecular endocrinology (Baltimore, Md.) |
Volume | 3 |
Issue number | 1 |
Pages (from-to) | 165-73 |
Number of pages | 9 |
ISSN | 0888-8809 |
Publication status | Published - Jan 1989 |
- Animals, Animals, Newborn, Bromodeoxyuridine, Cell Division, Cells, Cultured, Chromatography, High Pressure Liquid, Growth Hormone, Immunohistochemistry, Insulin, Islets of Langerhans, Proinsulin, Rats, Rats, Inbred Strains, Recombinant Proteins
Research areas
ID: 47974458