Good performance of an immunoassay based method for nevirapine measurements in human breast milk
Research output: Contribution to journal › Journal article › Research › peer-review
Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 µL) and no pre-treatment of the samples.
Original language | English |
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Journal | Clinical Chemistry and Laboratory Medicine |
Volume | 49 |
Issue number | 7 |
Pages (from-to) | 1171-5 |
Number of pages | 5 |
ISSN | 1434-6621 |
DOIs | |
Publication status | Published - 1 Jul 2011 |
ID: 40155073