Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis. / Ronco, Troels; Klaas, Ilka C.; Stegger, Marc; Svennesen, Line; Astrup, Lærke B.; Farre, Michael; Pedersen, Karl.

In: Veterinary Microbiology, Vol. 215, 2018, p. 35-42.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ronco, T, Klaas, IC, Stegger, M, Svennesen, L, Astrup, LB, Farre, M & Pedersen, K 2018, 'Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis', Veterinary Microbiology, vol. 215, pp. 35-42. https://doi.org/10.1016/j.vetmic.2018.01.003

APA

Ronco, T., Klaas, I. C., Stegger, M., Svennesen, L., Astrup, L. B., Farre, M., & Pedersen, K. (2018). Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis. Veterinary Microbiology, 215, 35-42. https://doi.org/10.1016/j.vetmic.2018.01.003

Vancouver

Ronco T, Klaas IC, Stegger M, Svennesen L, Astrup LB, Farre M et al. Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis. Veterinary Microbiology. 2018;215:35-42. https://doi.org/10.1016/j.vetmic.2018.01.003

Author

Ronco, Troels ; Klaas, Ilka C. ; Stegger, Marc ; Svennesen, Line ; Astrup, Lærke B. ; Farre, Michael ; Pedersen, Karl. / Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis. In: Veterinary Microbiology. 2018 ; Vol. 215. pp. 35-42.

Bibtex

@article{e03b2d471a184739b391842db0e21b21,
title = "Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis",
abstract = "Staphylococcus aureus is one of the most common pathogens that cause mastitis in dairy cows. Various subtypes, virulence genes and mobile genetic elements have been associated with isolates from bulk tank milk and clinical mastitis. So far, no Danish cattle associated S. aureus isolates have been whole-genome sequenced and further analyzed. Thus, the main objective was to investigate the population structure and genomic content of isolates from bulk tank milk and clinical mastitis, using whole-genome sequencing. This may reveal the origin of strains that cause clinical mastitis. S. aureus isolates from bulk tank milk (n = 94) and clinical mastitis (n = 63) were collected from 91 and 24 different farms, respectively and whole-genome sequenced. The genomic content was analyzed and a phylogenetic tree based on single nucleotide polymorphisms was constructed. In general, the isolates from both bulk tank milk and clinical mastitis were of similar genetic background. This suggests that dairy cows are natural carriers of the S. aureus subtypes that cause clinical mastitis if the right conditions are present and that a broad range of subtypes cause mastitis. A phylogenetic cluster that mostly consisted of ST151 isolates carried three mobile genetic elements that were primarily found in this group. The prevalence of resistance genes was generally low. However, the first ST398 methicillin resistant S. aureus isolate from a Danish dairy cow with clinical mastitis was detected.",
keywords = "Bulk tank milk, Clinical mastitis, Genomic analysis, S. aureus, Virulence and resistance genes",
author = "Troels Ronco and Klaas, {Ilka C.} and Marc Stegger and Line Svennesen and Astrup, {L{\ae}rke B.} and Michael Farre and Karl Pedersen",
year = "2018",
doi = "10.1016/j.vetmic.2018.01.003",
language = "English",
volume = "215",
pages = "35--42",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Genomic investigation of Staphylococcus aureus isolates from bulk tank milk and dairy cows with clinical mastitis

AU - Ronco, Troels

AU - Klaas, Ilka C.

AU - Stegger, Marc

AU - Svennesen, Line

AU - Astrup, Lærke B.

AU - Farre, Michael

AU - Pedersen, Karl

PY - 2018

Y1 - 2018

N2 - Staphylococcus aureus is one of the most common pathogens that cause mastitis in dairy cows. Various subtypes, virulence genes and mobile genetic elements have been associated with isolates from bulk tank milk and clinical mastitis. So far, no Danish cattle associated S. aureus isolates have been whole-genome sequenced and further analyzed. Thus, the main objective was to investigate the population structure and genomic content of isolates from bulk tank milk and clinical mastitis, using whole-genome sequencing. This may reveal the origin of strains that cause clinical mastitis. S. aureus isolates from bulk tank milk (n = 94) and clinical mastitis (n = 63) were collected from 91 and 24 different farms, respectively and whole-genome sequenced. The genomic content was analyzed and a phylogenetic tree based on single nucleotide polymorphisms was constructed. In general, the isolates from both bulk tank milk and clinical mastitis were of similar genetic background. This suggests that dairy cows are natural carriers of the S. aureus subtypes that cause clinical mastitis if the right conditions are present and that a broad range of subtypes cause mastitis. A phylogenetic cluster that mostly consisted of ST151 isolates carried three mobile genetic elements that were primarily found in this group. The prevalence of resistance genes was generally low. However, the first ST398 methicillin resistant S. aureus isolate from a Danish dairy cow with clinical mastitis was detected.

AB - Staphylococcus aureus is one of the most common pathogens that cause mastitis in dairy cows. Various subtypes, virulence genes and mobile genetic elements have been associated with isolates from bulk tank milk and clinical mastitis. So far, no Danish cattle associated S. aureus isolates have been whole-genome sequenced and further analyzed. Thus, the main objective was to investigate the population structure and genomic content of isolates from bulk tank milk and clinical mastitis, using whole-genome sequencing. This may reveal the origin of strains that cause clinical mastitis. S. aureus isolates from bulk tank milk (n = 94) and clinical mastitis (n = 63) were collected from 91 and 24 different farms, respectively and whole-genome sequenced. The genomic content was analyzed and a phylogenetic tree based on single nucleotide polymorphisms was constructed. In general, the isolates from both bulk tank milk and clinical mastitis were of similar genetic background. This suggests that dairy cows are natural carriers of the S. aureus subtypes that cause clinical mastitis if the right conditions are present and that a broad range of subtypes cause mastitis. A phylogenetic cluster that mostly consisted of ST151 isolates carried three mobile genetic elements that were primarily found in this group. The prevalence of resistance genes was generally low. However, the first ST398 methicillin resistant S. aureus isolate from a Danish dairy cow with clinical mastitis was detected.

KW - Bulk tank milk

KW - Clinical mastitis

KW - Genomic analysis

KW - S. aureus

KW - Virulence and resistance genes

U2 - 10.1016/j.vetmic.2018.01.003

DO - 10.1016/j.vetmic.2018.01.003

M3 - Journal article

C2 - 29426404

AN - SCOPUS:85041478253

VL - 215

SP - 35

EP - 42

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -

ID: 231412476