Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila

Research output: Contribution to journal › Journal article › Research › peer-review

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Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila. / Groth, Casper; Vaid, Pooja; Khatpe, Aditi; Prashali, Nelchi; Ahiya, Avantika; Andrejeva, Diana; Chakladar, Madhumita; Nagarkar, Sanket; Paul, Rachel; Kelkar, Devaki; Eichenlaub, Teresa; Herranz, Hector; Sridhar, T. S.; Cohen, Stephen M.; Shashidhara, L. S.

In: G3: Genes, Genomes, Genetics, Vol. 10, No. 9, 2020, p. 2999-3008.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Groth, C, Vaid, P, Khatpe, A, Prashali, N, Ahiya, A, Andrejeva, D, Chakladar, M, Nagarkar, S, Paul, R, Kelkar, D, Eichenlaub, T, Herranz, H, Sridhar, TS, Cohen, SM & Shashidhara, LS 2020, 'Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila', G3: Genes, Genomes, Genetics, vol. 10, no. 9, pp. 2999-3008. https://doi.org/10.1534/g3.120.401545

APA

Groth, C., Vaid, P., Khatpe, A., Prashali, N., Ahiya, A., Andrejeva, D., Chakladar, M., Nagarkar, S., Paul, R., Kelkar, D., Eichenlaub, T., Herranz, H., Sridhar, T. S., Cohen, S. M., & Shashidhara, L. S. (2020). Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila. G3: Genes, Genomes, Genetics, 10(9), 2999-3008. https://doi.org/10.1534/g3.120.401545

Vancouver

Groth C, Vaid P, Khatpe A, Prashali N, Ahiya A, Andrejeva D et al. Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila. G3: Genes, Genomes, Genetics. 2020;10(9):2999-3008. https://doi.org/10.1534/g3.120.401545

Author

Groth, Casper ; Vaid, Pooja ; Khatpe, Aditi ; Prashali, Nelchi ; Ahiya, Avantika ; Andrejeva, Diana ; Chakladar, Madhumita ; Nagarkar, Sanket ; Paul, Rachel ; Kelkar, Devaki ; Eichenlaub, Teresa ; Herranz, Hector ; Sridhar, T. S. ; Cohen, Stephen M. ; Shashidhara, L. S. / Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila. In: G3: Genes, Genomes, Genetics. 2020 ; Vol. 10, No. 9. pp. 2999-3008.

Bibtex

@article{9eabe0c5341b499c9cb6867ccb6ded86,
title = "Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila",
abstract = "Genetic approaches in Drosophila have successfully identified many genes involved in regulation of growth control as well as genetic interactions relevant to the initiation and progression of cancer in vivo. Here, we report on large-scale RNAi-based screens to identify potential tumor suppressor genes that interact with known cancer-drivers: the Epidermal Growth Factor Receptor and the Hippo pathway transcriptional cofactor Yorkie. These screens were designed to identify genes whose depletion drove tissue expressing EGFR or Yki from a state of benign overgrowth into neoplastic transformation in vivo. We also report on an independent screen aimed to identify genes whose depletion suppressed formation of neoplastic tumors in an existing EGFR-dependent neoplasia model. Many of the positives identified here are known to be functional in growth control pathways. We also find a number of novel connections to Yki and EGFR driven tissue growth, mostly unique to one of the two. Thus, resources provided here would be useful to all researchers who study negative regulators of growth during development and cancer in the context of activated EGFR and/or Yki and positive regulators of growth in the context of activated EGFR. Resources reported here are available freely for anyone to use.",
keywords = "Drosophila, EGFR, Hippo pathway, Neoplasia, Tumorigenesis",
author = "Casper Groth and Pooja Vaid and Aditi Khatpe and Nelchi Prashali and Avantika Ahiya and Diana Andrejeva and Madhumita Chakladar and Sanket Nagarkar and Rachel Paul and Devaki Kelkar and Teresa Eichenlaub and Hector Herranz and Sridhar, {T. S.} and Cohen, {Stephen M.} and Shashidhara, {L. S.}",
year = "2020",
doi = "10.1534/g3.120.401545",
language = "English",
volume = "10",
pages = "2999--3008",
journal = "G3: Genes, Genomes, Genetics (Bethesda)",
issn = "2160-1836",
publisher = "Genetics Society of America",
number = "9",

}

RIS

TY - JOUR

T1 - Genome-wide screen for context-dependent tumor suppressors identified using in vivo models for neoplasia in Drosophila

AU - Groth, Casper

AU - Vaid, Pooja

AU - Khatpe, Aditi

AU - Prashali, Nelchi

AU - Ahiya, Avantika

AU - Andrejeva, Diana

AU - Chakladar, Madhumita

AU - Nagarkar, Sanket

AU - Paul, Rachel

AU - Kelkar, Devaki

AU - Eichenlaub, Teresa

AU - Herranz, Hector

AU - Sridhar, T. S.

AU - Cohen, Stephen M.

AU - Shashidhara, L. S.

PY - 2020

Y1 - 2020

N2 - Genetic approaches in Drosophila have successfully identified many genes involved in regulation of growth control as well as genetic interactions relevant to the initiation and progression of cancer in vivo. Here, we report on large-scale RNAi-based screens to identify potential tumor suppressor genes that interact with known cancer-drivers: the Epidermal Growth Factor Receptor and the Hippo pathway transcriptional cofactor Yorkie. These screens were designed to identify genes whose depletion drove tissue expressing EGFR or Yki from a state of benign overgrowth into neoplastic transformation in vivo. We also report on an independent screen aimed to identify genes whose depletion suppressed formation of neoplastic tumors in an existing EGFR-dependent neoplasia model. Many of the positives identified here are known to be functional in growth control pathways. We also find a number of novel connections to Yki and EGFR driven tissue growth, mostly unique to one of the two. Thus, resources provided here would be useful to all researchers who study negative regulators of growth during development and cancer in the context of activated EGFR and/or Yki and positive regulators of growth in the context of activated EGFR. Resources reported here are available freely for anyone to use.

AB - Genetic approaches in Drosophila have successfully identified many genes involved in regulation of growth control as well as genetic interactions relevant to the initiation and progression of cancer in vivo. Here, we report on large-scale RNAi-based screens to identify potential tumor suppressor genes that interact with known cancer-drivers: the Epidermal Growth Factor Receptor and the Hippo pathway transcriptional cofactor Yorkie. These screens were designed to identify genes whose depletion drove tissue expressing EGFR or Yki from a state of benign overgrowth into neoplastic transformation in vivo. We also report on an independent screen aimed to identify genes whose depletion suppressed formation of neoplastic tumors in an existing EGFR-dependent neoplasia model. Many of the positives identified here are known to be functional in growth control pathways. We also find a number of novel connections to Yki and EGFR driven tissue growth, mostly unique to one of the two. Thus, resources provided here would be useful to all researchers who study negative regulators of growth during development and cancer in the context of activated EGFR and/or Yki and positive regulators of growth in the context of activated EGFR. Resources reported here are available freely for anyone to use.

KW - Drosophila

KW - EGFR

KW - Hippo pathway

KW - Neoplasia

KW - Tumorigenesis

U2 - 10.1534/g3.120.401545

DO - 10.1534/g3.120.401545

M3 - Journal article

C2 - 32737065

AN - SCOPUS:85090282336

VL - 10

SP - 2999

EP - 3008

JO - G3: Genes, Genomes, Genetics (Bethesda)

JF - G3: Genes, Genomes, Genetics (Bethesda)

SN - 2160-1836

IS - 9

ER -

ID: 249105961