Genome information processing by the INO80 chromatin remodeler positions nucleosomes
Research output: Contribution to journal › Journal article › Research › peer-review
The fundamental molecular determinants by which ATP-dependent chromatin remodelers organize nucleosomes across eukaryotic genomes remain largely elusive. Here, chromatin reconstitutions on physiological, whole-genome templates reveal how remodelers read and translate genomic information into nucleosome positions. Using the yeast genome and the multi-subunit INO80 remodeler as a paradigm, we identify DNA shape/mechanics encoded signature motifs as sufficient for nucleosome positioning and distinct from known DNA sequence preferences of histones. INO80 processes such information through an allosteric interplay between its core- and Arp8-modules that probes mechanical properties of nucleosomal and linker DNA. At promoters, INO80 integrates this readout of DNA shape/mechanics with a readout of co-evolved sequence motifs via interaction with general regulatory factors bound to these motifs. Our findings establish a molecular mechanism for robust and yet adjustable +1 nucleosome positioning and, more generally, remodelers as information processing hubs that enable active organization and allosteric regulation of the first level of chromatin.
Original language | English |
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Journal | Nature Communications |
Volume | 12 |
Issue number | 1 |
Pages (from-to) | 3231 |
ISSN | 2041-1723 |
DOIs | |
Publication status | Published - 28 May 2021 |
Externally published | Yes |
- Allosteric Regulation/genetics, Animals, Chromatin Assembly and Disassembly, DNA, Fungal/chemistry, Drosophila Proteins/metabolism, Drosophila melanogaster/genetics, Gene Expression Regulation, Genome, Fungal, Histones/genetics, Humans, Larva/genetics, Nucleic Acid Conformation, Nucleosomes/metabolism, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics
Research areas
ID: 301922050