Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1. / Chemla, Axel; Arena, Giuseppe; Onal, Gizem; Walter, Jonas; Berenguer-Escuder, Clara; Grossmann, Dajana; Grünewald, Anne; Schwamborn, Jens C.; Krüger, Rejko.
In: Stem Cell Research, Vol. 71, 103145, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1
AU - Chemla, Axel
AU - Arena, Giuseppe
AU - Onal, Gizem
AU - Walter, Jonas
AU - Berenguer-Escuder, Clara
AU - Grossmann, Dajana
AU - Grünewald, Anne
AU - Schwamborn, Jens C.
AU - Krüger, Rejko
N1 - Publisher Copyright: © 2023 The Author(s)
PY - 2023
Y1 - 2023
N2 - Fibroblasts from two Parkinson's disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).
AB - Fibroblasts from two Parkinson's disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).
U2 - 10.1016/j.scr.2023.103145
DO - 10.1016/j.scr.2023.103145
M3 - Journal article
C2 - 37364399
AN - SCOPUS:85162887901
VL - 71
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
M1 - 103145
ER -
ID: 366993347