Galanin: distribution and effect on contractile activity and release of vasoactive intestinal polypeptide from the isolated perfused porcine ileum
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Galanin : distribution and effect on contractile activity and release of vasoactive intestinal polypeptide from the isolated perfused porcine ileum. / Poulsen, Steen Seier; Holst, J J.
In: Digestion, Vol. 47, No. 4, 1990, p. 191-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Galanin
T2 - distribution and effect on contractile activity and release of vasoactive intestinal polypeptide from the isolated perfused porcine ileum
AU - Poulsen, Steen Seier
AU - Holst, J J
PY - 1990
Y1 - 1990
N2 - In the pig ileum galanin (GAL)-like immunoreactivity was identified in nerve cell bodies of the submucous plexus and in nerve fibers of the circular and longitudinal muscle layer. Infusion of 5.10(-10)-10(-8) M of GAL into the arterial line of the isolated perfused porcine ileum decreased the frequency of spontaneous phasic contractions in a dose-dependent manner. The frequency of phasic contractions during maximal inhibition by GAL 10(-8) M was 13 +/- 4% (mean +/- SE) of basal frequency (p less than 0.05). The recovery from inhibition by GAL 10(-8) M lasted 16 +/- 1 min. Tonic contractions were not observed in this experimental set-up, neither by standard perfused catheter manometry nor by measurement of cross-sectional area of an intraluminally located balloon. Infusion of GAL 10(-8) M decreased the venous release of vasoactive intestinal polypeptide to 80 +/- 8% of basal release (p less than 0.05). It is concluded that GAL may participate in the regulation of small intestinal motility in the pig.
AB - In the pig ileum galanin (GAL)-like immunoreactivity was identified in nerve cell bodies of the submucous plexus and in nerve fibers of the circular and longitudinal muscle layer. Infusion of 5.10(-10)-10(-8) M of GAL into the arterial line of the isolated perfused porcine ileum decreased the frequency of spontaneous phasic contractions in a dose-dependent manner. The frequency of phasic contractions during maximal inhibition by GAL 10(-8) M was 13 +/- 4% (mean +/- SE) of basal frequency (p less than 0.05). The recovery from inhibition by GAL 10(-8) M lasted 16 +/- 1 min. Tonic contractions were not observed in this experimental set-up, neither by standard perfused catheter manometry nor by measurement of cross-sectional area of an intraluminally located balloon. Infusion of GAL 10(-8) M decreased the venous release of vasoactive intestinal polypeptide to 80 +/- 8% of basal release (p less than 0.05). It is concluded that GAL may participate in the regulation of small intestinal motility in the pig.
KW - Animals
KW - Galanin
KW - Gastrointestinal Motility
KW - Ileum
KW - Neuropeptides
KW - Peptides
KW - Perfusion
KW - Radioimmunoassay
KW - Swine
KW - Vasoactive Intestinal Peptide
M3 - Journal article
C2 - 1712741
VL - 47
SP - 191
EP - 199
JO - Digestion
JF - Digestion
SN - 0012-2823
IS - 4
ER -
ID: 47488206