Standard
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. / Winzeler, E A; Shoemaker, D D; Astromoff, A; Liang, H; Anderson, K; Andre, B; Bangham, R; Benito, R; Boeke, J D; Bussey, H; Chu, A M; Connelly, C; Davis, K; Dietrich, F; Dow, S W; El Bakkoury, M; Foury, F; Friend, S H; Gentalen, E; Giaever, G; Hegemann, J H; Jones, T; Laub, M; Liao, H; Liebundguth, N; Lockhart, D J; Lucau-Danila, A; Lussier, M; M'Rabet, N; Menard, P; Mittmann, M; Pai, C; Rebischung, C; Revuelta, J L; Riles, L; Roberts, C J; Ross-MacDonald, P; Scherens, B; Snyder, M; Sookhai-Mahadeo, S; Storms, R K; Véronneau, S; Voet, M; Volckaert, G; Ward, T R; Wysocki, R; Yen, G S; Yu, K; Zimmermann, K; Philippsen, P; Johnston, M; Davis, R W.
In:
Science, Vol. 285, No. 5429, 1999, p. 901-6.
Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
Winzeler, EA, Shoemaker, DD, Astromoff, A, Liang, H, Anderson, K, Andre, B, Bangham, R, Benito, R, Boeke, JD, Bussey, H, Chu, AM, Connelly, C, Davis, K, Dietrich, F, Dow, SW, El Bakkoury, M, Foury, F, Friend, SH, Gentalen, E, Giaever, G, Hegemann, JH, Jones, T, Laub, M, Liao, H, Liebundguth, N, Lockhart, DJ, Lucau-Danila, A, Lussier, M, M'Rabet, N, Menard, P, Mittmann, M, Pai, C, Rebischung, C, Revuelta, JL, Riles, L, Roberts, CJ, Ross-MacDonald, P, Scherens, B, Snyder, M, Sookhai-Mahadeo, S, Storms, RK, Véronneau, S, Voet, M, Volckaert, G, Ward, TR, Wysocki, R, Yen, GS, Yu, K, Zimmermann, K, Philippsen, P, Johnston, M & Davis, RW 1999, 'Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis', Science, vol. 285, no. 5429, pp. 901-6.
APA
Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., Chu, A. M., Connelly, C., Davis, K., Dietrich, F., Dow, S. W., El Bakkoury, M., Foury, F., Friend, S. H., Gentalen, E., ... Davis, R. W. (1999). Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science, 285(5429), 901-6.
Vancouver
Winzeler EA, Shoemaker DD, Astromoff A, Liang H, Anderson K, Andre B et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science. 1999;285(5429):901-6.
Author
Winzeler, E A ; Shoemaker, D D ; Astromoff, A ; Liang, H ; Anderson, K ; Andre, B ; Bangham, R ; Benito, R ; Boeke, J D ; Bussey, H ; Chu, A M ; Connelly, C ; Davis, K ; Dietrich, F ; Dow, S W ; El Bakkoury, M ; Foury, F ; Friend, S H ; Gentalen, E ; Giaever, G ; Hegemann, J H ; Jones, T ; Laub, M ; Liao, H ; Liebundguth, N ; Lockhart, D J ; Lucau-Danila, A ; Lussier, M ; M'Rabet, N ; Menard, P ; Mittmann, M ; Pai, C ; Rebischung, C ; Revuelta, J L ; Riles, L ; Roberts, C J ; Ross-MacDonald, P ; Scherens, B ; Snyder, M ; Sookhai-Mahadeo, S ; Storms, R K ; Véronneau, S ; Voet, M ; Volckaert, G ; Ward, T R ; Wysocki, R ; Yen, G S ; Yu, K ; Zimmermann, K ; Philippsen, P ; Johnston, M ; Davis, R W. / Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. In: Science. 1999 ; Vol. 285, No. 5429. pp. 901-6.
Bibtex
@article{197625f05da311df928f000ea68e967b,
title = "Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis",
abstract = "The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.",
author = "Winzeler, {E A} and Shoemaker, {D D} and A Astromoff and H Liang and K Anderson and B Andre and R Bangham and R Benito and Boeke, {J D} and H Bussey and Chu, {A M} and C Connelly and K Davis and F Dietrich and Dow, {S W} and {El Bakkoury}, M and F Foury and Friend, {S H} and E Gentalen and G Giaever and Hegemann, {J H} and T Jones and M Laub and H Liao and N Liebundguth and Lockhart, {D J} and A Lucau-Danila and M Lussier and N M'Rabet and P Menard and M Mittmann and C Pai and C Rebischung and Revuelta, {J L} and L Riles and Roberts, {C J} and P Ross-MacDonald and B Scherens and M Snyder and S Sookhai-Mahadeo and Storms, {R K} and S V{\'e}ronneau and M Voet and G Volckaert and Ward, {T R} and R Wysocki and Yen, {G S} and K Yu and K Zimmermann and P Philippsen and M Johnston and Davis, {R W}",
note = "Keywords: Culture Media; Gene Deletion; Gene Expression Regulation, Fungal; Gene Targeting; Genes, Essential; Genes, Fungal; Genome, Fungal; Open Reading Frames; Phenotype; Polymerase Chain Reaction; Recombination, Genetic; Saccharomyces cerevisiae",
year = "1999",
language = "English",
volume = "285",
pages = "901--6",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5429",
}
RIS
TY - JOUR
T1 - Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis
AU - Winzeler, E A
AU - Shoemaker, D D
AU - Astromoff, A
AU - Liang, H
AU - Anderson, K
AU - Andre, B
AU - Bangham, R
AU - Benito, R
AU - Boeke, J D
AU - Bussey, H
AU - Chu, A M
AU - Connelly, C
AU - Davis, K
AU - Dietrich, F
AU - Dow, S W
AU - El Bakkoury, M
AU - Foury, F
AU - Friend, S H
AU - Gentalen, E
AU - Giaever, G
AU - Hegemann, J H
AU - Jones, T
AU - Laub, M
AU - Liao, H
AU - Liebundguth, N
AU - Lockhart, D J
AU - Lucau-Danila, A
AU - Lussier, M
AU - M'Rabet, N
AU - Menard, P
AU - Mittmann, M
AU - Pai, C
AU - Rebischung, C
AU - Revuelta, J L
AU - Riles, L
AU - Roberts, C J
AU - Ross-MacDonald, P
AU - Scherens, B
AU - Snyder, M
AU - Sookhai-Mahadeo, S
AU - Storms, R K
AU - Véronneau, S
AU - Voet, M
AU - Volckaert, G
AU - Ward, T R
AU - Wysocki, R
AU - Yen, G S
AU - Yu, K
AU - Zimmermann, K
AU - Philippsen, P
AU - Johnston, M
AU - Davis, R W
N1 - Keywords: Culture Media; Gene Deletion; Gene Expression Regulation, Fungal; Gene Targeting; Genes, Essential; Genes, Fungal; Genome, Fungal; Open Reading Frames; Phenotype; Polymerase Chain Reaction; Recombination, Genetic; Saccharomyces cerevisiae
PY - 1999
Y1 - 1999
N2 - The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.
AB - The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.
M3 - Journal article
C2 - 10436161
VL - 285
SP - 901
EP - 906
JO - Science
JF - Science
SN - 0036-8075
IS - 5429
ER -
