From pluripotency to totipotency: an experimentalist's guide to cellular potency

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From pluripotency to totipotency : an experimentalist's guide to cellular potency. / Riveiro, Alba Redo; Brickman, Joshua Mark.

In: Development, Vol. 147, No. 16, 189845, 08.2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Riveiro, AR & Brickman, JM 2020, 'From pluripotency to totipotency: an experimentalist's guide to cellular potency', Development, vol. 147, no. 16, 189845. https://doi.org/10.1242/dev.189845

APA

Riveiro, A. R., & Brickman, J. M. (2020). From pluripotency to totipotency: an experimentalist's guide to cellular potency. Development, 147(16), [189845]. https://doi.org/10.1242/dev.189845

Vancouver

Riveiro AR, Brickman JM. From pluripotency to totipotency: an experimentalist's guide to cellular potency. Development. 2020 Aug;147(16). 189845. https://doi.org/10.1242/dev.189845

Author

Riveiro, Alba Redo ; Brickman, Joshua Mark. / From pluripotency to totipotency : an experimentalist's guide to cellular potency. In: Development. 2020 ; Vol. 147, No. 16.

Bibtex

@article{411e705a8c9c4d64b0e267e7db42224c,
title = "From pluripotency to totipotency: an experimentalist's guide to cellular potency",
abstract = "Embryonic stem cells (ESCs) are derived from the pre-implantation mammalian blastocyst. At this point in time, the newly formed embryo is concerned with the generation and expansion of both the embryonic lineages required to build the embryo and the extra-embryonic lineages that support development. When used in grafting experiments, embryonic cells from early developmental stages can contribute to both embryonic and extra-embryonic lineages, but it is generally accepted that ESCs can give rise to only embryonic lineages. As a result, they are referred to as pluripotent, rather than totipotent. Here, we consider the experimental potential of various ESC populations and a number of recently identified in vitro culture systems producing states beyond pluripotency and reminiscent of those observed during pre-implantation development. We also consider the nature of totipotency and the extent to which cell populations in these culture systems exhibit this property.",
keywords = "Developmental biology, Pluripotency, Totipotency, EMBRYONIC STEM-CELLS, BLASTOCYST-LIKE STRUCTURES, PRIMITIVE ENDODERM, MOUSE EMBRYOS, GROUND-STATE, SELF-RENEWAL, LINEAGE SEGREGATION, 2-CELL EMBRYOS, FATE DECISIONS, INNER",
author = "Riveiro, {Alba Redo} and Brickman, {Joshua Mark}",
year = "2020",
month = aug,
doi = "10.1242/dev.189845",
language = "English",
volume = "147",
journal = "Development",
issn = "0950-1991",
publisher = "The Company of Biologists",
number = "16",

}

RIS

TY - JOUR

T1 - From pluripotency to totipotency

T2 - an experimentalist's guide to cellular potency

AU - Riveiro, Alba Redo

AU - Brickman, Joshua Mark

PY - 2020/8

Y1 - 2020/8

N2 - Embryonic stem cells (ESCs) are derived from the pre-implantation mammalian blastocyst. At this point in time, the newly formed embryo is concerned with the generation and expansion of both the embryonic lineages required to build the embryo and the extra-embryonic lineages that support development. When used in grafting experiments, embryonic cells from early developmental stages can contribute to both embryonic and extra-embryonic lineages, but it is generally accepted that ESCs can give rise to only embryonic lineages. As a result, they are referred to as pluripotent, rather than totipotent. Here, we consider the experimental potential of various ESC populations and a number of recently identified in vitro culture systems producing states beyond pluripotency and reminiscent of those observed during pre-implantation development. We also consider the nature of totipotency and the extent to which cell populations in these culture systems exhibit this property.

AB - Embryonic stem cells (ESCs) are derived from the pre-implantation mammalian blastocyst. At this point in time, the newly formed embryo is concerned with the generation and expansion of both the embryonic lineages required to build the embryo and the extra-embryonic lineages that support development. When used in grafting experiments, embryonic cells from early developmental stages can contribute to both embryonic and extra-embryonic lineages, but it is generally accepted that ESCs can give rise to only embryonic lineages. As a result, they are referred to as pluripotent, rather than totipotent. Here, we consider the experimental potential of various ESC populations and a number of recently identified in vitro culture systems producing states beyond pluripotency and reminiscent of those observed during pre-implantation development. We also consider the nature of totipotency and the extent to which cell populations in these culture systems exhibit this property.

KW - Developmental biology

KW - Pluripotency

KW - Totipotency

KW - EMBRYONIC STEM-CELLS

KW - BLASTOCYST-LIKE STRUCTURES

KW - PRIMITIVE ENDODERM

KW - MOUSE EMBRYOS

KW - GROUND-STATE

KW - SELF-RENEWAL

KW - LINEAGE SEGREGATION

KW - 2-CELL EMBRYOS

KW - FATE DECISIONS

KW - INNER

U2 - 10.1242/dev.189845

DO - 10.1242/dev.189845

M3 - Journal article

C2 - 32847824

VL - 147

JO - Development

JF - Development

SN - 0950-1991

IS - 16

M1 - 189845

ER -

ID: 256979418