Four newly isolated fuselloviruses from extreme geothermal environments reveal unusual morphologies and a possible interviral recombination mechanism
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Four newly isolated fuselloviruses from extreme geothermal environments reveal unusual morphologies and a possible interviral recombination mechanism. / Redder, Peter; Peng, Xu; Brügger, Kim; Shah, Shiraz Ali; Roesch, Ferdinand; Greve, Bo; She, Qunxin; Schleper, Christa; Forterre, Patrick; Garrett, Roger A; Prangishvili, David.
In: Environmental Microbiology, Vol. 11, No. 11, 2009, p. 2849-62.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Four newly isolated fuselloviruses from extreme geothermal environments reveal unusual morphologies and a possible interviral recombination mechanism
AU - Redder, Peter
AU - Peng, Xu
AU - Brügger, Kim
AU - Shah, Shiraz Ali
AU - Roesch, Ferdinand
AU - Greve, Bo
AU - She, Qunxin
AU - Schleper, Christa
AU - Forterre, Patrick
AU - Garrett, Roger A
AU - Prangishvili, David
PY - 2009
Y1 - 2009
N2 - Spindle-shaped virus-like particles are abundant in extreme geothermal environments, from which five spindle-shaped viral species have been isolated to date. They infect members of the hyperthermophilic archaeal genus Sulfolobus, and constitute the Fuselloviridae, a family of double-stranded DNA viruses. Here we present four new members of this family, all from terrestrial acidic hot springs. Two of the new viruses exhibit a novel morphotype for their proposed attachment structures, and specific features of their genome sequences strongly suggest the identity of the host-attachment protein. All fuselloviral genomes are highly conserved at the nucleotide level, although the regions of conservation differ between virus-pairs, consistent with a high frequency of homologous recombination having occurred between them. We propose a fuselloviral specific mechanism for interviral recombination, and show that the spacers of the Sulfolobus CRISPR antiviral system are not biased to the highly similar regions of the fusellovirus genomes.
AB - Spindle-shaped virus-like particles are abundant in extreme geothermal environments, from which five spindle-shaped viral species have been isolated to date. They infect members of the hyperthermophilic archaeal genus Sulfolobus, and constitute the Fuselloviridae, a family of double-stranded DNA viruses. Here we present four new members of this family, all from terrestrial acidic hot springs. Two of the new viruses exhibit a novel morphotype for their proposed attachment structures, and specific features of their genome sequences strongly suggest the identity of the host-attachment protein. All fuselloviral genomes are highly conserved at the nucleotide level, although the regions of conservation differ between virus-pairs, consistent with a high frequency of homologous recombination having occurred between them. We propose a fuselloviral specific mechanism for interviral recombination, and show that the spacers of the Sulfolobus CRISPR antiviral system are not biased to the highly similar regions of the fusellovirus genomes.
U2 - 10.1111/j.1462-2920.2009.02009.x
DO - 10.1111/j.1462-2920.2009.02009.x
M3 - Journal article
C2 - 19638177
VL - 11
SP - 2849
EP - 2862
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2912
IS - 11
ER -
ID: 15637568