Fluorescence in situ hybridization for detecting Coxiella burnetii in tissue samples from chronic Q fever patients
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Fluorescence in situ hybridization for detecting Coxiella burnetii in tissue samples from chronic Q fever patients. / Buijs, Sheila B.; Weehuizen, Jesper M.; Jensen, Tim Kåre; Boye, Mette; Hermans, Mirjam HA.; Nooijen, Peet TGA.; Hoepelman, Andy IM.; Bleeker-Rovers, Chantal P.; Oosterheert, Jan Jelrik; Wever, Peter C.
In: Clinical Microbiology and Infection, Vol. 28, No. 11, 2022, p. 1502.e1-1502.e5.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Fluorescence in situ hybridization for detecting Coxiella burnetii in tissue samples from chronic Q fever patients
AU - Buijs, Sheila B.
AU - Weehuizen, Jesper M.
AU - Jensen, Tim Kåre
AU - Boye, Mette
AU - Hermans, Mirjam HA.
AU - Nooijen, Peet TGA.
AU - Hoepelman, Andy IM.
AU - Bleeker-Rovers, Chantal P.
AU - Oosterheert, Jan Jelrik
AU - Wever, Peter C.
PY - 2022
Y1 - 2022
N2 - ObjectiveDetection of the intracellular bacterium Coxiella burnetii, causative agent of chronic Q fever, is notoriously difficult. Diagnosis of and duration of antibiotic treatment for chronic Q fever is partly determined by detection of the bacterium with polymerase chain reaction (PCR). Fluorescence in situ hybridization (FISH) might be a promising technique for detecting C. burnetii in tissue samples from chronic Q fever patients, but its value in comparison with PCR is uncertain. We aim to assess the value of FISH for detecting C. burnetii in tissue of chronic Q fever patients.MethodsFISH and PCR were performed on tissue samples from Dutch chronic Q fever patients collected during surgery or autopsy. Sensitivity, specificity, and overall diagnostic accuracy were calculated. Additionally, data on patient and disease characteristics were collected from electronic medical records.ResultsIn total, 49 tissue samples from mainly vascular walls, heart valves, or placentas, obtained from 39 chronic Q fever patients, were examined by FISH and PCR. The sensitivity and specificity of FISH compared to PCR for detecting C. burnetii in tissue samples from chronic Q fever patients was 45.2% (95% confidence interval (CI), 27.3% – 64.0%) and 84.6% (95% CI, 54.6% – 98.1%), respectively. The overall diagnostic accuracy was 56.8% (95% CI, 42.2% - 72.3%). Two C. burnetii PCR negative placentas were FISH positive. Four FISH results (8.2%) were deemed inconclusive because of autofluorescence.ConclusionWith an overall diagnostic accuracy of 57.8%, we conclude that FISH has limited value in the routine diagnostics of chronic Q fever.
AB - ObjectiveDetection of the intracellular bacterium Coxiella burnetii, causative agent of chronic Q fever, is notoriously difficult. Diagnosis of and duration of antibiotic treatment for chronic Q fever is partly determined by detection of the bacterium with polymerase chain reaction (PCR). Fluorescence in situ hybridization (FISH) might be a promising technique for detecting C. burnetii in tissue samples from chronic Q fever patients, but its value in comparison with PCR is uncertain. We aim to assess the value of FISH for detecting C. burnetii in tissue of chronic Q fever patients.MethodsFISH and PCR were performed on tissue samples from Dutch chronic Q fever patients collected during surgery or autopsy. Sensitivity, specificity, and overall diagnostic accuracy were calculated. Additionally, data on patient and disease characteristics were collected from electronic medical records.ResultsIn total, 49 tissue samples from mainly vascular walls, heart valves, or placentas, obtained from 39 chronic Q fever patients, were examined by FISH and PCR. The sensitivity and specificity of FISH compared to PCR for detecting C. burnetii in tissue samples from chronic Q fever patients was 45.2% (95% confidence interval (CI), 27.3% – 64.0%) and 84.6% (95% CI, 54.6% – 98.1%), respectively. The overall diagnostic accuracy was 56.8% (95% CI, 42.2% - 72.3%). Two C. burnetii PCR negative placentas were FISH positive. Four FISH results (8.2%) were deemed inconclusive because of autofluorescence.ConclusionWith an overall diagnostic accuracy of 57.8%, we conclude that FISH has limited value in the routine diagnostics of chronic Q fever.
U2 - 10.1016/j.cmi.2022.06.015
DO - 10.1016/j.cmi.2022.06.015
M3 - Journal article
C2 - 35724869
VL - 28
SP - 1502.e1-1502.e5
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
SN - 1198-743X
IS - 11
ER -
ID: 339134077