Ficolin-3-mediated lectin complement pathway activation in patients with subarachnoid hemorrhage
Research output: Contribution to journal › Journal article › Research › peer-review
OBJECTIVES: To assess the involvement of ficolin-3, the main initiator of the lectin complement pathway (LCP), in subarachnoid hemorrhage (SAH) pathology and outcome.
METHODS: In this preliminary exploratory study, plasma concentration of ficolin-3 and of ficolin-3-mediated functional LCP activity was measured, along with that of other LCP initiators (mannose-binding lectin, ficolin-2, and ficolin-1), C3 activation products, and soluble C5b-9 terminal complex, in a prospective cohort of 39 patients with SAH and 20 healthy controls. The following parameters were recorded: SAH severity, assessed using the World Federation of Neurosurgical Societies grading scale; vasospasm, defined as neuro-worsening with angiographic confirmation of vessel narrowing; cerebral ischemia, defined as hypodense lesion on CT scan performed before discharge; and 6-month outcome, assessed using the Glasgow Outcome Scale.
RESULTS: In patients, no changes were detected for ficolin-3 compared with controls. Notably, however, ficolin-3-mediated functional LCP activity was reduced. Low levels of plasma ficolin-3 and ficolin-3-mediated functional LCP activity were related to SAH severity, vasospasm, and cerebral ischemia. Moreover, ficolin-3 functional LCP activity was decreased in patients with unfavorable outcome.
CONCLUSION: Our data provide evidence that LCP is activated after SAH and that the actual plasma concentrations of ficolin-3 reflect the severity of brain injury as evaluated by clinical and structural parameters. These results support the idea that ficolin-3-mediated functional LCP activity may be targeted to control injury progression in SAH.
Original language | English |
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Journal | Neurology |
Volume | 82 |
Issue number | 2 |
Pages (from-to) | 126-134 |
Number of pages | 9 |
ISSN | 0028-3878 |
DOIs | |
Publication status | Published - 14 Jan 2014 |
- Aged, Brain Ischemia, Cohort Studies, Complement Pathway, Mannose-Binding Lectin, Enzyme-Linked Immunosorbent Assay, Female, Glycoproteins, Humans, Lectins, Male, Middle Aged, Neurosurgical Procedures, Prospective Studies, Subarachnoid Hemorrhage, Tomography, X-Ray Computed, Treatment Outcome, Vasospasm, Intracranial
Research areas
ID: 138308669