Extraction of [99mTc]-d,l-HM-PAO across the blood-brain barrier
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Extraction of [99mTc]-d,l-HM-PAO across the blood-brain barrier. / Andersen, A R; Friberg, H; Knudsen, K B; Barry, D I; Paulson, O B; Schmidt, J F; Lassen, N A; Neirinckx, R D.
In: Journal of Cerebral Blood Flow and Metabolism, Vol. 8, No. 6, 12.1988, p. S44-51.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Extraction of [99mTc]-d,l-HM-PAO across the blood-brain barrier
AU - Andersen, A R
AU - Friberg, H
AU - Knudsen, K B
AU - Barry, D I
AU - Paulson, O B
AU - Schmidt, J F
AU - Lassen, N A
AU - Neirinckx, R D
PY - 1988/12
Y1 - 1988/12
N2 - The initial extraction (E) across the blood-brain barrier (BBB) of [99mTc]-d,l-HM-PAO after intracarotid injection was measured in 14 Wistar rats and 6 patients using the double indicator, single injection method with Na-24 as the cotracer. In both series, cerebral blood flow (CBF) was measured using the initial slope of the xenon-133 washout curve after intracarotid bolus injection. In rats, bolus size (20 or 120 microliters), bolus type (saline or 10% albumin), or CBF were changed. First-pass extraction was dependent on CBF (p less than 0.001): With a small bolus of saline and at resting CBF (0.75 ml/g/min), E was 0.81, decreasing to 0.56 at a high CBF (1.5 ml/g/min). The calculated permeability surface area product (PS) increased linearly from 1.2 to 1.5 ml/g/min when CBF increased from 0.8 to 1.5 ml/g/min (p less than 0.01). E was found to increase when the bolus volume of saline was increased from 20 to 120 microliters, while using a 120 microliters bolus containing 10% albumin resulted in a decrease in E. This suggests that HM-PAO binding to albumin is not totally and rapidly reversible during a single passage through brain capillaries and that binding to blood elements may reduce the apparent extraction across brain capillaries. In patients using a bolus of 1 ml saline, E decreased linearly with increasing CBF (r = -0.81, p less than 0.001). For a CBF of 0.59 ml/g/min and an average apparent E of 0.72, an apparent PS product of 0.76 ml/g/min was calculated.(ABSTRACT TRUNCATED AT 250 WORDS)
AB - The initial extraction (E) across the blood-brain barrier (BBB) of [99mTc]-d,l-HM-PAO after intracarotid injection was measured in 14 Wistar rats and 6 patients using the double indicator, single injection method with Na-24 as the cotracer. In both series, cerebral blood flow (CBF) was measured using the initial slope of the xenon-133 washout curve after intracarotid bolus injection. In rats, bolus size (20 or 120 microliters), bolus type (saline or 10% albumin), or CBF were changed. First-pass extraction was dependent on CBF (p less than 0.001): With a small bolus of saline and at resting CBF (0.75 ml/g/min), E was 0.81, decreasing to 0.56 at a high CBF (1.5 ml/g/min). The calculated permeability surface area product (PS) increased linearly from 1.2 to 1.5 ml/g/min when CBF increased from 0.8 to 1.5 ml/g/min (p less than 0.01). E was found to increase when the bolus volume of saline was increased from 20 to 120 microliters, while using a 120 microliters bolus containing 10% albumin resulted in a decrease in E. This suggests that HM-PAO binding to albumin is not totally and rapidly reversible during a single passage through brain capillaries and that binding to blood elements may reduce the apparent extraction across brain capillaries. In patients using a bolus of 1 ml saline, E decreased linearly with increasing CBF (r = -0.81, p less than 0.001). For a CBF of 0.59 ml/g/min and an average apparent E of 0.72, an apparent PS product of 0.76 ml/g/min was calculated.(ABSTRACT TRUNCATED AT 250 WORDS)
KW - Animals
KW - Blood-Brain Barrier
KW - Brain/metabolism
KW - Cerebrovascular Circulation
KW - Humans
KW - Male
KW - Organometallic Compounds/analysis
KW - Oximes/analysis
KW - Rats
KW - Rats, Inbred Strains
KW - Technetium/analysis
KW - Technetium Tc 99m Exametazime
U2 - 10.1038/jcbfm.1988.32
DO - 10.1038/jcbfm.1988.32
M3 - Journal article
C2 - 3192642
VL - 8
SP - S44-51
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 6
ER -
ID: 275604360