External thoracic duct-venous shunt in conscious pigs for long term studies of connective tissue metabolites in lymph
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External thoracic duct-venous shunt in conscious pigs for long term studies of connective tissue metabolites in lymph. / Jensen, L T; Olesen, H P; Risteli, J; Lorenzen, I.
In: J Anim Sci, Vol. 40, No. 6, 11.1990, p. 620-4.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - External thoracic duct-venous shunt in conscious pigs for long term studies of connective tissue metabolites in lymph
AU - Jensen, L T
AU - Olesen, H P
AU - Risteli, J
AU - Lorenzen, I
PY - 1990/11
Y1 - 1990/11
N2 - An experimental animal model for lymph studies is described. Thoracic duct-venous shunt was established in 12 pigs. Shunt patency averaged 5.5 days. The composition of connective tissue metabolites in lymph and serum were investigated during a standardized surgical operation (thoracotomy) under general anesthesia. We measured the carboxyterminal propeptide of type I procollagen (PICP), the aminoterminal propeptide of type III procollagen (PIIINP) hyaluronan (HA) and total protein. During surgery/anesthesia lymph PICP (p less than 0.04), lymph PIIINP (p less than 0.03) and serum PIIINP (p less than 0.01) and serum PIIINP (p less than 0.03) increased. The changes may be explained by the inactive physical state of the animals. HA showed wide variations, with a tendency like PIIINP. In conscious animals the lymph/serum ratio of PIIINP and HA were 10 and 35, respectively, indicating that lymph is a major route of tissue clearance for these components. The lymph/serum ratio of PICP was 1.0 in conscious pigs, indicating a direct release into the circulation. Total protein in lymph decreased (p less than 0.04) during surgery/anesthesia, whereas no changes were observed in serum. Pigs can be used instead of dogs and sheep in studies on lymph. The effect of surgery/anesthesia must be taken into consideration.
AB - An experimental animal model for lymph studies is described. Thoracic duct-venous shunt was established in 12 pigs. Shunt patency averaged 5.5 days. The composition of connective tissue metabolites in lymph and serum were investigated during a standardized surgical operation (thoracotomy) under general anesthesia. We measured the carboxyterminal propeptide of type I procollagen (PICP), the aminoterminal propeptide of type III procollagen (PIIINP) hyaluronan (HA) and total protein. During surgery/anesthesia lymph PICP (p less than 0.04), lymph PIIINP (p less than 0.03) and serum PIIINP (p less than 0.01) and serum PIIINP (p less than 0.03) increased. The changes may be explained by the inactive physical state of the animals. HA showed wide variations, with a tendency like PIIINP. In conscious animals the lymph/serum ratio of PIIINP and HA were 10 and 35, respectively, indicating that lymph is a major route of tissue clearance for these components. The lymph/serum ratio of PICP was 1.0 in conscious pigs, indicating a direct release into the circulation. Total protein in lymph decreased (p less than 0.04) during surgery/anesthesia, whereas no changes were observed in serum. Pigs can be used instead of dogs and sheep in studies on lymph. The effect of surgery/anesthesia must be taken into consideration.
KW - Anastomosis, Surgical
KW - Animals
KW - Hyaluronic Acid
KW - Lymph
KW - Procollagen
KW - Swine
KW - Thoracic Duct
KW - Thoracotomy
KW - Time Factors
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 2172627
VL - 40
SP - 620
EP - 624
JO - Laboratory animal science (Chicago)
JF - Laboratory animal science (Chicago)
SN - 0023-6764
IS - 6
ER -
ID: 168533505