Expression of the epidermal growth factor system in human endometrium during the menstrual cycle
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Expression of the epidermal growth factor system in human endometrium during the menstrual cycle. / Ejskjaer, Kirsten; Sørensen, B S; Poulsen, Steen Seier; Mogensen, O; Forman, Axel; Nexø, Ebba.
In: Molecular Human Reproduction, Vol. 11, No. 8, 08.2005, p. 543-51.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Expression of the epidermal growth factor system in human endometrium during the menstrual cycle
AU - Ejskjaer, Kirsten
AU - Sørensen, B S
AU - Poulsen, Steen Seier
AU - Mogensen, O
AU - Forman, Axel
AU - Nexø, Ebba
PY - 2005/8
Y1 - 2005/8
N2 - The epidermal growth factor (EGF) system is ubiquitous in humans and plays fundamental roles in embryogenesis, development, proliferation and differentiation. As the endometrium of fertile women is characterized by proliferation and differentiation, we hypothesize a role for the EGF system. Fourteen premenopausal women had endometrial samples removed on day 6 +/- 1 and day 6 +/- 1 and 12 +/- 1 after ovulation during one menstrual cycle. RNA was extracted and analysed by real-time PCR, and immunohistochemistry was performed to localize the components of the EGF system. Human EGF Receptor 1 (HER1) showed highest expression during the proliferative phase, HER2 and HER4 during the early and HER3 during the late secretory phase. Amphiregulin (AR) and transforming growth factor alpha (TGFalpha) expression is highest in proliferative phase. Heparin binding (HB)-EGF and betacellulin (BCL) show no variation. Epiregulin (EP) is detectable in some samples. EGF is undetectable. HER1, HER2, HER3 and HER4 were localized to the epithelium and glands HER3 and HER4 solely in the secretory phase. Amphiregulin was seen in leucocytes and stromal cells, TGFalpha and betacellulin in the epithelial lining, epiregulin in stromal cells whereas HB-EGF and EGF are undetectable. In conclusions, we observed cyclical expression of the four EGF receptors and two ligands and localized all four receptors and four ligands in endometrial biopsies. This suggests a role for the EGF system in growth of the endometrium.
AB - The epidermal growth factor (EGF) system is ubiquitous in humans and plays fundamental roles in embryogenesis, development, proliferation and differentiation. As the endometrium of fertile women is characterized by proliferation and differentiation, we hypothesize a role for the EGF system. Fourteen premenopausal women had endometrial samples removed on day 6 +/- 1 and day 6 +/- 1 and 12 +/- 1 after ovulation during one menstrual cycle. RNA was extracted and analysed by real-time PCR, and immunohistochemistry was performed to localize the components of the EGF system. Human EGF Receptor 1 (HER1) showed highest expression during the proliferative phase, HER2 and HER4 during the early and HER3 during the late secretory phase. Amphiregulin (AR) and transforming growth factor alpha (TGFalpha) expression is highest in proliferative phase. Heparin binding (HB)-EGF and betacellulin (BCL) show no variation. Epiregulin (EP) is detectable in some samples. EGF is undetectable. HER1, HER2, HER3 and HER4 were localized to the epithelium and glands HER3 and HER4 solely in the secretory phase. Amphiregulin was seen in leucocytes and stromal cells, TGFalpha and betacellulin in the epithelial lining, epiregulin in stromal cells whereas HB-EGF and EGF are undetectable. In conclusions, we observed cyclical expression of the four EGF receptors and two ligands and localized all four receptors and four ligands in endometrial biopsies. This suggests a role for the EGF system in growth of the endometrium.
KW - Adolescent
KW - Adult
KW - DNA Primers
KW - Endometrium
KW - Epidermal Growth Factor
KW - Female
KW - Gene Expression Regulation
KW - Humans
KW - Immunohistochemistry
KW - Menstrual Cycle
KW - RNA, Messenger
KW - Receptor, Epidermal Growth Factor
KW - Transforming Growth Factor alpha
U2 - 10.1093/molehr/gah207
DO - 10.1093/molehr/gah207
M3 - Journal article
C2 - 16100239
VL - 11
SP - 543
EP - 551
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
SN - 1360-9947
IS - 8
ER -
ID: 47486175