Experimental Salmonella typhimurium infections in rats. III. Transfer of immunity with primed lymphocyte subpopulations
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Experimental Salmonella typhimurium infections in rats. III. Transfer of immunity with primed lymphocyte subpopulations. / Hougen, H P; Jensen, E T.
In: APMIS: Acta Pathologica, Microbiologica et Immunologica Scandinavica, Vol. 98, 11.1990, p. 1015-21.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Experimental Salmonella typhimurium infections in rats. III. Transfer of immunity with primed lymphocyte subpopulations
AU - Hougen, H P
AU - Jensen, E T
PY - 1990/11
Y1 - 1990/11
N2 - The protective effect of primed lymphocytes against a lethal dose of Salmonella typhimurium was studied in athymic and euthymic LEW rats. Primed lymphocytes were obtained by inoculating euthymic and thymus-grafted animals with a non-lethal dose of Salmonella typhimurium. Four weeks after the infection, spleen lymphocytes were separated by panning technique and antibody-coated magnetic microsphere separation using antibodies to pan T and pan B lymphocytes and subsequent sorting in a fluorescence activated cell sorter by means of monoclonal antibodies against CD4+ and CD8+ cells. Euthymic and athymic rats were injected with different doses of primed pan B, pan T, CD4+ and CD8+ T lymphocytes before inoculation with a lethal bacterial dose. Most of the animals treated with pan B, pan T or CD8+ cells died within two weeks. After treatment with primed CD4+ cells, only six of 39 animals died. Doses as low as 10(4) cells from both euthymic or thymus-grafted animals were effective, and athymic and euthymic recipients survived equally well. Four weeks after the infection both athymic and euthymic animals housed very few bacteria and had high antibacterial antibody titres. The percentages of splenic and lymph node CD4+ cells in the athymic rats were comparable to those found in the euthymic animals. The study shows that primed CD4+ lymphocytes even in very low doses are able to induce immunity against a Salmonella typhimurium infection.
AB - The protective effect of primed lymphocytes against a lethal dose of Salmonella typhimurium was studied in athymic and euthymic LEW rats. Primed lymphocytes were obtained by inoculating euthymic and thymus-grafted animals with a non-lethal dose of Salmonella typhimurium. Four weeks after the infection, spleen lymphocytes were separated by panning technique and antibody-coated magnetic microsphere separation using antibodies to pan T and pan B lymphocytes and subsequent sorting in a fluorescence activated cell sorter by means of monoclonal antibodies against CD4+ and CD8+ cells. Euthymic and athymic rats were injected with different doses of primed pan B, pan T, CD4+ and CD8+ T lymphocytes before inoculation with a lethal bacterial dose. Most of the animals treated with pan B, pan T or CD8+ cells died within two weeks. After treatment with primed CD4+ cells, only six of 39 animals died. Doses as low as 10(4) cells from both euthymic or thymus-grafted animals were effective, and athymic and euthymic recipients survived equally well. Four weeks after the infection both athymic and euthymic animals housed very few bacteria and had high antibacterial antibody titres. The percentages of splenic and lymph node CD4+ cells in the athymic rats were comparable to those found in the euthymic animals. The study shows that primed CD4+ lymphocytes even in very low doses are able to induce immunity against a Salmonella typhimurium infection.
KW - Animals
KW - CD4-Positive T-Lymphocytes
KW - Dose-Response Relationship, Immunologic
KW - Immunization, Passive
KW - Mice
KW - Mice, Nude
KW - Rats
KW - Rats, Inbred Lew
KW - Salmonella Infections, Animal
KW - Salmonella typhimurium
KW - T-Lymphocyte Subsets
KW - T-Lymphocytes, Regulatory
M3 - Journal article
C2 - 1979006
VL - 98
SP - 1015
EP - 1021
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
ER -
ID: 44514397