Experimental porcine neosporosis
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Experimental porcine neosporosis. / Jensen, Lene; Jensen, Tim K.; Lind, Peter; Henriksen, Svend Aage; Uggla, Arvid; Bille-Hansen, Vivi.
In: APMIS, Vol. 106, No. 4, 1998, p. 475-482.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Experimental porcine neosporosis
AU - Jensen, Lene
AU - Jensen, Tim K.
AU - Lind, Peter
AU - Henriksen, Svend Aage
AU - Uggla, Arvid
AU - Bille-Hansen, Vivi
PY - 1998
Y1 - 1998
N2 - Six gilts were inoculated intramuscularly with 2.5x106 tachyzoites of Neospora caninum on three different days of gestation to study the pathogenic effect of Neospora infection in pigs, including possible transplacental transmission. The gilts were euthanized 59, 30, and 9/10 days postinoculation (.p.i.), corresponding to days 107, 102/106 and 110/111 of pregnancy. With the exception of one animal (euthanized day 9 p.i.) all gilts seroconverted as measured by the indirect, fluorescent antibody test (IFAT). Neosporosis with multifocal intralobular necrotizing hepatitis was seen in the two gilts inoculated 9/10 days before euthanasia. The uterus of one gilt inoculated 59 days before euthanasia revealed granulomatous and focal necrotizing endometritis with a corresponding multifocal necrosis of the trophoblasts of two fetuses. Transplacental neosporosis was indicated in the two fetuses by strongly elevated Neospora IFAT titres in pleural fluid and by the presence of multifocal necrotizing encephalitis and hepatitis together with non- suppurative myocarditis, pneumonitis, nephritis and hepatitis. Furthermore, N. caninum was re-isolated in cell culture from one of these fetuses. A third fetus from the same gilt revealed only disseminated, pinpoint necroses in the liver. Immunohistochemically, N. caninum tachyzoites were detected in association with histopathological changes in the liver and the endometrium of the gilts, and in the brain, liver, and allantochorion of the three fetuses.
AB - Six gilts were inoculated intramuscularly with 2.5x106 tachyzoites of Neospora caninum on three different days of gestation to study the pathogenic effect of Neospora infection in pigs, including possible transplacental transmission. The gilts were euthanized 59, 30, and 9/10 days postinoculation (.p.i.), corresponding to days 107, 102/106 and 110/111 of pregnancy. With the exception of one animal (euthanized day 9 p.i.) all gilts seroconverted as measured by the indirect, fluorescent antibody test (IFAT). Neosporosis with multifocal intralobular necrotizing hepatitis was seen in the two gilts inoculated 9/10 days before euthanasia. The uterus of one gilt inoculated 59 days before euthanasia revealed granulomatous and focal necrotizing endometritis with a corresponding multifocal necrosis of the trophoblasts of two fetuses. Transplacental neosporosis was indicated in the two fetuses by strongly elevated Neospora IFAT titres in pleural fluid and by the presence of multifocal necrotizing encephalitis and hepatitis together with non- suppurative myocarditis, pneumonitis, nephritis and hepatitis. Furthermore, N. caninum was re-isolated in cell culture from one of these fetuses. A third fetus from the same gilt revealed only disseminated, pinpoint necroses in the liver. Immunohistochemically, N. caninum tachyzoites were detected in association with histopathological changes in the liver and the endometrium of the gilts, and in the brain, liver, and allantochorion of the three fetuses.
KW - Experimental
KW - Neospora caninum
KW - Pathology
KW - Pigs
KW - Serology
KW - Transplacental transmission
UR - http://www.scopus.com/inward/record.url?scp=0031775801&partnerID=8YFLogxK
U2 - 10.1111/j.1699-0463.1998.tb01374.x
DO - 10.1111/j.1699-0463.1998.tb01374.x
M3 - Journal article
C2 - 9637270
AN - SCOPUS:0031775801
VL - 106
SP - 475
EP - 482
JO - Acta Pathologica et Microbiologica Scandinavica - Section A Pathology
JF - Acta Pathologica et Microbiologica Scandinavica - Section A Pathology
SN - 0365-4184
IS - 4
ER -
ID: 339896589