Evidence for a mediator cycle at the initiation of transcription
Research output: Contribution to journal › Journal article › Research › peer-review
Free and elongating (DNA-bound) forms of RNA polymerase II were separated from yeast. Most cellular polymerase II was found in the elongating fraction, which contained all enzyme phosphorylated on the C-terminal domain and none of the 15-subunit mediator of transcriptional regulation. These and other findings suggest that mediator enters and leaves initiation complexes during every round of transcription, in a process that may be coupled to C- terminal domain phosphorylation.
Original language | English |
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Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 94 |
Issue number | 12 |
Pages (from-to) | 6075-6078 |
Number of pages | 4 |
ISSN | 0027-8424 |
DOIs | |
Publication status | Published - 10 Jun 1997 |
Externally published | Yes |
- C-terminal domain, RNA polymerase II, Yeast
Research areas
ID: 331574765