Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania. / Jørgensen, A; Shao, Jing; Maselle, S; Yangi, E; Thomsen, A; Matunda, S; Bygbjerg, Ib Christian; Gøtzsche, P; Svendsen, J; Skinhøj.
In: Scandinavian Journal of Infectious Diseases, Vol. 22, No. 3, 01.01.1990, p. 283-5.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania
AU - Jørgensen, A
AU - Shao, Jing
AU - Maselle, S
AU - Yangi, E
AU - Thomsen, A
AU - Matunda, S
AU - Bygbjerg, Ib Christian
AU - Gøtzsche, P
AU - Svendsen, J
AU - Skinhøj, null
PY - 1990/1/1
Y1 - 1990/1/1
N2 - 200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.
AB - 200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.
KW - Blotting, Western
KW - Enzyme-Linked Immunosorbent Assay
KW - False Negative Reactions
KW - False Positive Reactions
KW - HIV Antibodies
KW - HIV Infections
KW - Humans
KW - Observer Variation
KW - Predictive Value of Tests
KW - Tanzania
M3 - Journal article
C2 - 2371543
VL - 22
SP - 283
EP - 285
JO - Infectious Diseases
JF - Infectious Diseases
SN - 2374-4235
IS - 3
ER -
ID: 33890974