Environmental spread of microbes impacts the development of metabolic phenotypes in mice transplanted with microbial communities from humans
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Environmental spread of microbes impacts the development of metabolic phenotypes in mice transplanted with microbial communities from humans. / Zhang, Li; Bahl, Martin Iain; Roager, Henrik Munch; Fonvig, Cilius Esmann; Hellgren, Lars I; Frandsen, Henrik Lauritz; Pedersen, Oluf; Holm, Jens-Christian; Hansen, Torben; Licht, Tine Rask.
In: The ISME Journal, Vol. 11, 18.11.2017, p. 676-690.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Environmental spread of microbes impacts the development of metabolic phenotypes in mice transplanted with microbial communities from humans
AU - Zhang, Li
AU - Bahl, Martin Iain
AU - Roager, Henrik Munch
AU - Fonvig, Cilius Esmann
AU - Hellgren, Lars I
AU - Frandsen, Henrik Lauritz
AU - Pedersen, Oluf
AU - Holm, Jens-Christian
AU - Hansen, Torben
AU - Licht, Tine Rask
PY - 2017/11/18
Y1 - 2017/11/18
N2 - Microbiota transplantation to germ-free animals is a powerful method to study involvement of gut microbes in the aetiology of metabolic syndrome. Owing to large interpersonal variability in gut microbiota, studies with broad coverage of donors are needed to elucidate the establishment of human-derived microbiotas in mice, factors affecting this process and resulting impact on metabolic health. We thus transplanted faecal microbiotas from humans (16 obese and 16 controls) separately into 64 germ-free Swiss Webster mice caged in pairs within four isolators, with two isolators assigned to each phenotype, thereby allowing us to explore the extent of microbial spread between cages in a well-controlled environment. Despite high group-wise similarity between obese and control human microbiotas, transplanted mice in the four isolators developed distinct gut bacterial composition and activity, body mass gain, and insulin resistance. Spread of microbes between cages within isolators interacted with establishment of the transplanted microbiotas in mice, and contributed to the transmission of metabolic phenotypes. Our findings highlight the impact of donor variability and reveal that inter-individual spread of microbes contributes to the development of metabolic traits. This is of major importance for design of animal studies, and indicates that environmental transfer of microbes between individuals may affect host metabolic traits.The ISME Journal advance online publication, 18 November 2016; doi:10.1038/ismej.2016.151.
AB - Microbiota transplantation to germ-free animals is a powerful method to study involvement of gut microbes in the aetiology of metabolic syndrome. Owing to large interpersonal variability in gut microbiota, studies with broad coverage of donors are needed to elucidate the establishment of human-derived microbiotas in mice, factors affecting this process and resulting impact on metabolic health. We thus transplanted faecal microbiotas from humans (16 obese and 16 controls) separately into 64 germ-free Swiss Webster mice caged in pairs within four isolators, with two isolators assigned to each phenotype, thereby allowing us to explore the extent of microbial spread between cages in a well-controlled environment. Despite high group-wise similarity between obese and control human microbiotas, transplanted mice in the four isolators developed distinct gut bacterial composition and activity, body mass gain, and insulin resistance. Spread of microbes between cages within isolators interacted with establishment of the transplanted microbiotas in mice, and contributed to the transmission of metabolic phenotypes. Our findings highlight the impact of donor variability and reveal that inter-individual spread of microbes contributes to the development of metabolic traits. This is of major importance for design of animal studies, and indicates that environmental transfer of microbes between individuals may affect host metabolic traits.The ISME Journal advance online publication, 18 November 2016; doi:10.1038/ismej.2016.151.
U2 - 10.1038/ismej.2016.151
DO - 10.1038/ismej.2016.151
M3 - Journal article
C2 - 27858930
VL - 11
SP - 676
EP - 690
JO - I S M E Journal
JF - I S M E Journal
SN - 1751-7362
ER -
ID: 172765490