Engineered CHO cells for production of diverse, homogeneous glycoproteins
Research output: Contribution to journal › Journal article › Research › peer-review
Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.
Original language | English |
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Journal | Nature Biotechnology |
Volume | 33 |
Issue number | 8 |
Pages (from-to) | 842-844 |
Number of pages | 3 |
ISSN | 1087-0156 |
DOIs | |
Publication status | Published - 20 Jul 2015 |
ID: 143666578