Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects. / Gliemann, Lasse; Rytter, Nicolai; Lindskrog, Mads; Slingsby, Martina Helena Lundberg; Åkerström, Thorbjörn; Sylow, Lykke; Richter, Erik A.; Hellsten, Ylva.
In: Journal of Physiology, Vol. 595, No. 16, 2017, p. 5557-5571.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects
AU - Gliemann, Lasse
AU - Rytter, Nicolai
AU - Lindskrog, Mads
AU - Slingsby, Martina Helena Lundberg
AU - Åkerström, Thorbjörn
AU - Sylow, Lykke
AU - Richter, Erik A.
AU - Hellsten, Ylva
N1 - CURIS 2017 NEXS 222
PY - 2017
Y1 - 2017
N2 - Endothelial mechanotransduction is important for vascular function but alterations and activation of vascular mechanosensory proteins have not been investigated in humans. In endothelial cell culture, simple sugars effectively impair mechanosensor proteins. To study mechanosensor- and vascular function in humans, twelve young healthy male subjects supplemented their diet with 3 × 75 g sucrose day(-1) for 14 days in a randomized cross-over design. Before and after the intervention period, the hyperemic response to passive lower leg movement and active knee extensor exercise was determined by ultrasound doppler. A muscle biopsy was obtained from the thigh muscle before and after acute passive leg movement, to asses the protein amount and phosphorylation status of mechanosensory proteins and NADPH oxidase. The sucrose intervention led to a reduced flow response to passive movement (by 17 ± 2 %) and to 12 watts of active exercise (by 9 ± 1 %), indicating impaired vascular function. Reduced flow response to passive and active exercise was paralleled by a significant upregulation of Platelet endothelial cell adhesion molecule (PECAM-1), endothelial nitric oxide synthase, NADPH oxidase and the Rho family GTPase Rac1 protein expression in the muscle tissue as well as an increased basal phosphorylation status of Vascular endothelial growth factor receptor 2 and a reduced phosphorylation status of PECAM-1. The phosphorylation status was not acutely altered with passive leg movement. These findings indicate that regular intake of high levels of sucrose can impair vascular mechanotransduction, increase the oxidative stress potential and suggest that dietary excessive sugar intake may contribute to the development of vascular disease. This article is protected by copyright. All rights reserved.
AB - Endothelial mechanotransduction is important for vascular function but alterations and activation of vascular mechanosensory proteins have not been investigated in humans. In endothelial cell culture, simple sugars effectively impair mechanosensor proteins. To study mechanosensor- and vascular function in humans, twelve young healthy male subjects supplemented their diet with 3 × 75 g sucrose day(-1) for 14 days in a randomized cross-over design. Before and after the intervention period, the hyperemic response to passive lower leg movement and active knee extensor exercise was determined by ultrasound doppler. A muscle biopsy was obtained from the thigh muscle before and after acute passive leg movement, to asses the protein amount and phosphorylation status of mechanosensory proteins and NADPH oxidase. The sucrose intervention led to a reduced flow response to passive movement (by 17 ± 2 %) and to 12 watts of active exercise (by 9 ± 1 %), indicating impaired vascular function. Reduced flow response to passive and active exercise was paralleled by a significant upregulation of Platelet endothelial cell adhesion molecule (PECAM-1), endothelial nitric oxide synthase, NADPH oxidase and the Rho family GTPase Rac1 protein expression in the muscle tissue as well as an increased basal phosphorylation status of Vascular endothelial growth factor receptor 2 and a reduced phosphorylation status of PECAM-1. The phosphorylation status was not acutely altered with passive leg movement. These findings indicate that regular intake of high levels of sucrose can impair vascular mechanotransduction, increase the oxidative stress potential and suggest that dietary excessive sugar intake may contribute to the development of vascular disease. This article is protected by copyright. All rights reserved.
KW - Vascular function
KW - Passive leg movement
KW - Mechanosensor
U2 - 10.1113/JP274623
DO - 10.1113/JP274623
M3 - Journal article
C2 - 28620941
VL - 595
SP - 5557
EP - 5571
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - 16
ER -
ID: 179559744