Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation
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Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation. / Gilbert, Christopher; Kristjuhan, Arnold; Winkler, G. Sebastiaan; Svejstrup, Jesper Q.
In: Molecular Cell, Vol. 14, No. 4, 21.05.2004, p. 457-464.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation
AU - Gilbert, Christopher
AU - Kristjuhan, Arnold
AU - Winkler, G. Sebastiaan
AU - Svejstrup, Jesper Q.
N1 - Funding Information: This project was supported by grants from Cancer Research UK and the European Union (HPRN-CT-2000-00087; to J.Q.S.) and by an EMBO Long-Term Fellowship (to G.S.W.). We thank Dirk Görlich for a generous gift of anti-Cbp80 antibodies.
PY - 2004/5/21
Y1 - 2004/5/21
N2 - The histone acetyltransferase Elongator was originally isolated as a component of the elongating form of RNA polymerase II (RNAPII) and a plethora of data has since supported a role for the factor in transcription. However, recent data has suggested that it is predominantly cytoplasmic and does not associate with the DNA of transcribed genes in vivo. Here, we report that Elongator binds to RNA both in vitro and in vivo. Using a modified chromatin immunoprecipitation procedure, RNA immunoprecipitation (RIP), we show that Elongator is indeed present at several actively transcribed genes and that it associates with the nascent RNA emanating from elongating RNAPII along the entire coding region of a gene. These results strongly support a role for Elongator in transcript elongation.
AB - The histone acetyltransferase Elongator was originally isolated as a component of the elongating form of RNA polymerase II (RNAPII) and a plethora of data has since supported a role for the factor in transcription. However, recent data has suggested that it is predominantly cytoplasmic and does not associate with the DNA of transcribed genes in vivo. Here, we report that Elongator binds to RNA both in vitro and in vivo. Using a modified chromatin immunoprecipitation procedure, RNA immunoprecipitation (RIP), we show that Elongator is indeed present at several actively transcribed genes and that it associates with the nascent RNA emanating from elongating RNAPII along the entire coding region of a gene. These results strongly support a role for Elongator in transcript elongation.
U2 - 10.1016/S1097-2765(04)00239-4
DO - 10.1016/S1097-2765(04)00239-4
M3 - Journal article
C2 - 15149595
AN - SCOPUS:2442425954
VL - 14
SP - 457
EP - 464
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 4
ER -
ID: 331040832