Elevated miR-9 in Cerebrospinal Fluid Is Associated with Poor Functional Outcome After Subarachnoid Hemorrhage
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Elevated miR-9 in Cerebrospinal Fluid Is Associated with Poor Functional Outcome After Subarachnoid Hemorrhage. / Bache, Søren; Rasmussen, Rune; Wolcott, Zoe; Rossing, Maria; Møgelvang, Rasmus; Tolnai, Daniel; Hassager, Christian; Forman, Julie L.; Køber, Lars; Nielsen, Finn Cilius; Kimberly, William T.; Møller, Kirsten.
In: Translational Stroke Research, Vol. 11, 2020, p. 1243–1252.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Elevated miR-9 in Cerebrospinal Fluid Is Associated with Poor Functional Outcome After Subarachnoid Hemorrhage
AU - Bache, Søren
AU - Rasmussen, Rune
AU - Wolcott, Zoe
AU - Rossing, Maria
AU - Møgelvang, Rasmus
AU - Tolnai, Daniel
AU - Hassager, Christian
AU - Forman, Julie L.
AU - Køber, Lars
AU - Nielsen, Finn Cilius
AU - Kimberly, William T.
AU - Møller, Kirsten
PY - 2020
Y1 - 2020
N2 - This study evaluated microRNA (miRNA) changes in cerebrospinal fluid (CSF) and their association with the occurrence of delayed cerebral ischemia (DCI) and poor functional outcome after SAH. Forty-three selected miRNAs were measured in daily CSF samples from a discovery cohort of SAH patients admitted to Rigshospitalet, Copenhagen, Denmark, and compared with neurologically healthy patients. Findings were validated in CSF from a replication cohort of SAH patients admitted to Massachusetts General Hospital, Boston, Massachusetts. The CSF levels of miRNA over time were compared with the occurrence of DCI, and functional outcome after 3 months. miRNAs were quantified in 427 CSF samples from 63 SAH patients in the discovery cohort, in 104 CSF samples from 63 SAH patients in the replication cohort, and in 11 CSF samples from 11 neurologically healthy patients. The miRNA profile changed remarkably immediately after SAH. Elevated miR-9-3p was associated with a poor functional outcome in the discovery cohort (p < 0.0001) after correction for multiple testing (q < 0.01) and in the replication cohort (p < 0.01). Furthermore, elevated miR-9-5p was associated with a poor functional outcome in the discovery cohort (p < 0.01) after correction for multiple testing (q < 0.05). No miRNA was associated with DCI in both cohorts. miR-9-3p and miR-9-5p are elevated in the CSF following SAH and this elevation is associated with a poor functional outcome. These elevations have potential roles in the progression of cerebral injury and could add to early prognostication.
AB - This study evaluated microRNA (miRNA) changes in cerebrospinal fluid (CSF) and their association with the occurrence of delayed cerebral ischemia (DCI) and poor functional outcome after SAH. Forty-three selected miRNAs were measured in daily CSF samples from a discovery cohort of SAH patients admitted to Rigshospitalet, Copenhagen, Denmark, and compared with neurologically healthy patients. Findings were validated in CSF from a replication cohort of SAH patients admitted to Massachusetts General Hospital, Boston, Massachusetts. The CSF levels of miRNA over time were compared with the occurrence of DCI, and functional outcome after 3 months. miRNAs were quantified in 427 CSF samples from 63 SAH patients in the discovery cohort, in 104 CSF samples from 63 SAH patients in the replication cohort, and in 11 CSF samples from 11 neurologically healthy patients. The miRNA profile changed remarkably immediately after SAH. Elevated miR-9-3p was associated with a poor functional outcome in the discovery cohort (p < 0.0001) after correction for multiple testing (q < 0.01) and in the replication cohort (p < 0.01). Furthermore, elevated miR-9-5p was associated with a poor functional outcome in the discovery cohort (p < 0.01) after correction for multiple testing (q < 0.05). No miRNA was associated with DCI in both cohorts. miR-9-3p and miR-9-5p are elevated in the CSF following SAH and this elevation is associated with a poor functional outcome. These elevations have potential roles in the progression of cerebral injury and could add to early prognostication.
KW - Cerebrospinal fluid
KW - MicroRNA
KW - Stroke
KW - Subarachnoid hemorrhage
U2 - 10.1007/s12975-020-00793-1
DO - 10.1007/s12975-020-00793-1
M3 - Journal article
C2 - 32248435
AN - SCOPUS:85082931626
VL - 11
SP - 1243
EP - 1252
JO - Translational Stroke Research
JF - Translational Stroke Research
SN - 1868-4483
ER -
ID: 240005304