Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons. / Untiet, Verena; Moeller, Lisa M; Ibarra-Soria, Ximena; Sánchez-Andrade, Gabriela; Stricker, Miriam; Neuhaus, Eva M; Logan, Darren W; Gensch, Thomas; Spehr, Marc.

In: Chemical Senses, Vol. 41, No. 8, 2016, p. 669-676.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Untiet, V, Moeller, LM, Ibarra-Soria, X, Sánchez-Andrade, G, Stricker, M, Neuhaus, EM, Logan, DW, Gensch, T & Spehr, M 2016, 'Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons', Chemical Senses, vol. 41, no. 8, pp. 669-676. https://doi.org/10.1093/chemse/bjw077

APA

Untiet, V., Moeller, L. M., Ibarra-Soria, X., Sánchez-Andrade, G., Stricker, M., Neuhaus, E. M., Logan, D. W., Gensch, T., & Spehr, M. (2016). Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons. Chemical Senses, 41(8), 669-676. https://doi.org/10.1093/chemse/bjw077

Vancouver

Untiet V, Moeller LM, Ibarra-Soria X, Sánchez-Andrade G, Stricker M, Neuhaus EM et al. Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons. Chemical Senses. 2016;41(8):669-676. https://doi.org/10.1093/chemse/bjw077

Author

Untiet, Verena ; Moeller, Lisa M ; Ibarra-Soria, Ximena ; Sánchez-Andrade, Gabriela ; Stricker, Miriam ; Neuhaus, Eva M ; Logan, Darren W ; Gensch, Thomas ; Spehr, Marc. / Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons. In: Chemical Senses. 2016 ; Vol. 41, No. 8. pp. 669-676.

Bibtex

@article{7c5c97bd4a9e42daa96bd7f012c1272a,
title = "Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons",
abstract = "In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca(2+)-activated Cl(-) current. As the physiological role of such a current is a direct function of the Cl(-) equilibrium potential, we determined the intracellular Cl(-) concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl(-)-sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl(-) levels in dendritic knobs, a substantially lower Cl(-) concentration in vomeronasal sustentacular cells, and an apparent Cl(-) gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca(2+)-activated Cl(-) channels.",
keywords = "Animals, Calcium/metabolism, Chloride Channels/metabolism, Chlorides/analysis, Cytosol/chemistry, Dendrites/chemistry, Mice, Sensory Receptor Cells/chemistry, Vomeronasal Organ/chemistry",
author = "Verena Untiet and Moeller, {Lisa M} and Ximena Ibarra-Soria and Gabriela S{\'a}nchez-Andrade and Miriam Stricker and Neuhaus, {Eva M} and Logan, {Darren W} and Thomas Gensch and Marc Spehr",
year = "2016",
doi = "10.1093/chemse/bjw077",
language = "English",
volume = "41",
pages = "669--676",
journal = "Chemical Senses",
issn = "0379-864X",
publisher = "Oxford University Press",
number = "8",

}

RIS

TY - JOUR

T1 - Elevated Cytosolic Cl- Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

AU - Untiet, Verena

AU - Moeller, Lisa M

AU - Ibarra-Soria, Ximena

AU - Sánchez-Andrade, Gabriela

AU - Stricker, Miriam

AU - Neuhaus, Eva M

AU - Logan, Darren W

AU - Gensch, Thomas

AU - Spehr, Marc

PY - 2016

Y1 - 2016

N2 - In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca(2+)-activated Cl(-) current. As the physiological role of such a current is a direct function of the Cl(-) equilibrium potential, we determined the intracellular Cl(-) concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl(-)-sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl(-) levels in dendritic knobs, a substantially lower Cl(-) concentration in vomeronasal sustentacular cells, and an apparent Cl(-) gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca(2+)-activated Cl(-) channels.

AB - In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca(2+)-activated Cl(-) current. As the physiological role of such a current is a direct function of the Cl(-) equilibrium potential, we determined the intracellular Cl(-) concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl(-)-sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl(-) levels in dendritic knobs, a substantially lower Cl(-) concentration in vomeronasal sustentacular cells, and an apparent Cl(-) gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca(2+)-activated Cl(-) channels.

KW - Animals

KW - Calcium/metabolism

KW - Chloride Channels/metabolism

KW - Chlorides/analysis

KW - Cytosol/chemistry

KW - Dendrites/chemistry

KW - Mice

KW - Sensory Receptor Cells/chemistry

KW - Vomeronasal Organ/chemistry

U2 - 10.1093/chemse/bjw077

DO - 10.1093/chemse/bjw077

M3 - Journal article

C2 - 27377750

VL - 41

SP - 669

EP - 676

JO - Chemical Senses

JF - Chemical Senses

SN - 0379-864X

IS - 8

ER -

ID: 209898840