Electrophoretic demonstration of glycoproteins, lipoproteins, and phosphoproteins in human and bovine enamel
Research output: Contribution to journal › Journal article › Research › peer-review
Enamel proteins from fully mineralized human molars and from bovine tooth germs were separated by electrophoresis. The gels were stained for detection of glycoproteins, lipoproteins, and phosphoproteins. Glycoproteins were shown by periodic acid-Schiff staining and lectin blotting. In mature human enamel a number of high molecular weight proteins could be demonstrated after ethylenediaminetetra-acetic acid demineralization and subsequent Triton X-100 extraction. These proteins are suggested to be lipoproteins. Phosphoproteins could only be visualized in enamel matrix from the tooth germs.
Original language | English |
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Journal | Caries Research |
Volume | 24 |
Issue number | 3 |
Pages (from-to) | 169-73 |
Number of pages | 4 |
ISSN | 0008-6568 |
Publication status | Published - 1990 |
Bibliographical note
Keywords: Animals; Cattle; Dental Enamel Proteins; Electrophoresis, Polyacrylamide Gel; Glycoproteins; Humans; Incisor; Lipoproteins; Periodic Acid-Schiff Reaction; Phosphoproteins; Wheat Germ Agglutinins
ID: 10141191