Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain
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Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain. / Nazerai, Loulieta; Buus, Søren; Stryhn, Anette; Thomsen, Allan Randrup; Christensen, Jan Pravsgaard.
In: Viruses, Vol. 13, No. 11, 2215, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain
AU - Nazerai, Loulieta
AU - Buus, Søren
AU - Stryhn, Anette
AU - Thomsen, Allan Randrup
AU - Christensen, Jan Pravsgaard
N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement, which represents an ongoing concern in the flavivirus-vaccinology field. In this report, we investigated the vaccination potential of replication-deficient adenoviral vectors encoding the ZIKV non-structural proteins 1 and 2 (NS1/NS2) and employed the strategy of linking the antigens to the MHC-II associated invariant chain (li) to improve immunogenicity and by inference, the level of protection. We demonstrated that li-linkage enhanced the production of anti-NS1 antibodies and induced an accelerated and prolonged polyfunctional CD8 T cell response in mice, which ultimately resulted in a high degree of protection against ZIKV infection of the CNS.
AB - It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement, which represents an ongoing concern in the flavivirus-vaccinology field. In this report, we investigated the vaccination potential of replication-deficient adenoviral vectors encoding the ZIKV non-structural proteins 1 and 2 (NS1/NS2) and employed the strategy of linking the antigens to the MHC-II associated invariant chain (li) to improve immunogenicity and by inference, the level of protection. We demonstrated that li-linkage enhanced the production of anti-NS1 antibodies and induced an accelerated and prolonged polyfunctional CD8 T cell response in mice, which ultimately resulted in a high degree of protection against ZIKV infection of the CNS.
KW - CD8 T cell response
KW - MHC-II invariant chain
KW - NS1/NS2
KW - Vaccine
KW - Zika virus
U2 - 10.3390/v13112215
DO - 10.3390/v13112215
M3 - Journal article
C2 - 34835021
AN - SCOPUS:85118742247
VL - 13
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 11
M1 - 2215
ER -
ID: 285311839