Effect of K+ and K+ channel blockers on pancreatic HCO3-transport.
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Effect of K+ and K+ channel blockers on pancreatic HCO3-transport. / Novak, I.
In: Zeitschrift für Gastroenterologie, Vol. 25 Suppl 3, 08.1987, p. 97-101.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of K+ and K+ channel blockers on pancreatic HCO3-transport.
AU - Novak, I.
PY - 1987/8
Y1 - 1987/8
N2 - The present study investigated whether there is a K+ conductive pathway on the basolateral membrane of pancreatic duct cells. Small inter- and intralobular ducts were isolated from rat pancreas and perfused in vitro. Duct cells were impaled and the potential across the basolateral membrane (PDbl) was measured. The resting potential was -61 +/- 1 mV. Ouabain (3 mmol/l) depolarized PDbl by 11 mV. Increase in the bath K+ concentration from 5 to 20 mmol/l depolarized PDbl by 15-16 mV. The effect of K+ channel blockers was tested. Ba2+ (1 mmol/l) added to the bath depolarized PDbl by 23-26 mV, whereas TEA+ (50 mmol/l) depolarized PDbl only by 7-8 mV. Dibutyryl cyclic AMP (0.5 mmol/l) depolarized PDbl by 20-30 mV. The results show that on the basolateral membrane of pancreatic duct cells there is a (Na+ + K+)-ATPase and a K+ conductive pathway sensitive to Ba2+.
AB - The present study investigated whether there is a K+ conductive pathway on the basolateral membrane of pancreatic duct cells. Small inter- and intralobular ducts were isolated from rat pancreas and perfused in vitro. Duct cells were impaled and the potential across the basolateral membrane (PDbl) was measured. The resting potential was -61 +/- 1 mV. Ouabain (3 mmol/l) depolarized PDbl by 11 mV. Increase in the bath K+ concentration from 5 to 20 mmol/l depolarized PDbl by 15-16 mV. The effect of K+ channel blockers was tested. Ba2+ (1 mmol/l) added to the bath depolarized PDbl by 23-26 mV, whereas TEA+ (50 mmol/l) depolarized PDbl only by 7-8 mV. Dibutyryl cyclic AMP (0.5 mmol/l) depolarized PDbl by 20-30 mV. The results show that on the basolateral membrane of pancreatic duct cells there is a (Na+ + K+)-ATPase and a K+ conductive pathway sensitive to Ba2+.
UR - http://www.scopus.com/inward/record.url?scp=18544392352&partnerID=8YFLogxK
M3 - Journal article
C2 - 2444037
AN - SCOPUS:18544392352
VL - 25 Suppl 3
SP - 97
EP - 101
JO - Zeitschrift fur Gastroenterologie
JF - Zeitschrift fur Gastroenterologie
SN - 0044-2771
ER -
ID: 240419973