Drug distribution in spinal cord during administration with spinal loop dialysis probes in anaesthetized rats
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Drug distribution in spinal cord during administration with spinal loop dialysis probes in anaesthetized rats. / Uustalu, Maria; Abelson, Klas S P.
In: Basic & Clinical Pharmacology & Toxicology, Vol. 100, No. 3, 03.2007, p. 196-200.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Drug distribution in spinal cord during administration with spinal loop dialysis probes in anaesthetized rats
AU - Uustalu, Maria
AU - Abelson, Klas S P
PY - 2007/3
Y1 - 2007/3
N2 - The present investigation aimed to study two methodological concerns of an experimental model, where a spinal loop dialysis probe is used for administration of substances to the spinal cord and sampling of neurotransmitters by microdialysis from the same area of anaesthetized rats. [(3)H]Epibatidine in concentrations of 1, 10 and 100 nM was dissolved in Ringer's solution and administered through the dialysis membrane into the dorsal region of the cervical spinal cord. First, the outflow of [(3)H]epibatidine from the probe into the spinal cord was examined with respect to different concentrations and changes over time. Then, the distribution of the different [(3)H]epibatidine concentrations along the spinal cord was studied. It was found that the percentage of [(3)H]epibatidine entering the spinal cord did not differ between different administered concentrations after a stabilization period of 60 min. The administered [(3)H]epibatidine was found to be distributed to the area closest to the dialysis probe and not dispersed along the spinal cord, and the distribution was equal for all concentrations. The data presented in this investigation provide information, which is important for interpretation of data from intraspinal administration of substances through the spinal loop dialysis probe.
AB - The present investigation aimed to study two methodological concerns of an experimental model, where a spinal loop dialysis probe is used for administration of substances to the spinal cord and sampling of neurotransmitters by microdialysis from the same area of anaesthetized rats. [(3)H]Epibatidine in concentrations of 1, 10 and 100 nM was dissolved in Ringer's solution and administered through the dialysis membrane into the dorsal region of the cervical spinal cord. First, the outflow of [(3)H]epibatidine from the probe into the spinal cord was examined with respect to different concentrations and changes over time. Then, the distribution of the different [(3)H]epibatidine concentrations along the spinal cord was studied. It was found that the percentage of [(3)H]epibatidine entering the spinal cord did not differ between different administered concentrations after a stabilization period of 60 min. The administered [(3)H]epibatidine was found to be distributed to the area closest to the dialysis probe and not dispersed along the spinal cord, and the distribution was equal for all concentrations. The data presented in this investigation provide information, which is important for interpretation of data from intraspinal administration of substances through the spinal loop dialysis probe.
KW - Analgesics, Non-Narcotic
KW - Animals
KW - Bicyclo Compounds, Heterocyclic
KW - Dose-Response Relationship, Drug
KW - Injections, Spinal
KW - Isotonic Solutions
KW - Male
KW - Microdialysis
KW - Models, Animal
KW - Pyridines
KW - Rats
KW - Rats, Sprague-Dawley
KW - Spinal Cord
KW - Tissue Distribution
U2 - 10.1111/j.1742-7843.2006.00034.x
DO - 10.1111/j.1742-7843.2006.00034.x
M3 - Journal article
C2 - 17309524
VL - 100
SP - 196
EP - 200
JO - Basic and Clinical Pharmacology and Toxicology
JF - Basic and Clinical Pharmacology and Toxicology
SN - 1742-7835
IS - 3
ER -
ID: 48010360