DNA conformational analysis in solution by uranyl mediated photocleavage

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

DNA conformational analysis in solution by uranyl mediated photocleavage. / Nielsen, Peter E.; Møllegaard, N E; Jeppesen, C.

In: Nucleic Acids Research, Vol. 18, No. 13, 1990, p. 3847-51.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, PE, Møllegaard, NE & Jeppesen, C 1990, 'DNA conformational analysis in solution by uranyl mediated photocleavage', Nucleic Acids Research, vol. 18, no. 13, pp. 3847-51.

APA

Nielsen, P. E., Møllegaard, N. E., & Jeppesen, C. (1990). DNA conformational analysis in solution by uranyl mediated photocleavage. Nucleic Acids Research, 18(13), 3847-51.

Vancouver

Nielsen PE, Møllegaard NE, Jeppesen C. DNA conformational analysis in solution by uranyl mediated photocleavage. Nucleic Acids Research. 1990;18(13):3847-51.

Author

Nielsen, Peter E. ; Møllegaard, N E ; Jeppesen, C. / DNA conformational analysis in solution by uranyl mediated photocleavage. In: Nucleic Acids Research. 1990 ; Vol. 18, No. 13. pp. 3847-51.

Bibtex

@article{15bed7c0e60011ddbf70000ea68e967b,
title = "DNA conformational analysis in solution by uranyl mediated photocleavage",
abstract = "Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.",
author = "Nielsen, {Peter E.} and M{\o}llegaard, {N E} and C Jeppesen",
note = "Keywords: Animals; Base Sequence; DNA; Densitometry; Molecular Sequence Data; Nucleic Acid Conformation; Photochemistry; Uranium; Uranyl Nitrate; Xenopus",
year = "1990",
language = "English",
volume = "18",
pages = "3847--51",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "13",

}

RIS

TY - JOUR

T1 - DNA conformational analysis in solution by uranyl mediated photocleavage

AU - Nielsen, Peter E.

AU - Møllegaard, N E

AU - Jeppesen, C

N1 - Keywords: Animals; Base Sequence; DNA; Densitometry; Molecular Sequence Data; Nucleic Acid Conformation; Photochemistry; Uranium; Uranyl Nitrate; Xenopus

PY - 1990

Y1 - 1990

N2 - Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.

AB - Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.

M3 - Journal article

C2 - 2374711

VL - 18

SP - 3847

EP - 3851

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -

ID: 9828913