Distribution of the UV filter 3-benzylidene camphor in rat following topical application
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A straightforward analytical method for determination of 3-benzylidene camphor (3-BC) in rat adipose tissue, brain, liver, muscle, plasma and testis following topical application was developed and validated. Three exposure levels (60, 180 and 540 mg kg-1 day-1) were tested for 65 days in male Sprague-Dawley rats (24 days postnatal). Sample preparation involving homogenization and n-heptane or methanol extraction of the tissue was applied before injection into the LC-ESI-MS-MS system. The response was linear from 2 to 100 μg l-1 for the qualifier and the quantifier MRM transitions (R2 (quantifier) > 0.994). Detection limit of the method corresponded to 0.005 μg g-1 tissue and 12.5 μg l-1 plasma, respectively. Recovery was determined for all tissues (adipose tissue: 40%; all other tissues: 80-100%) at three individual levels. 3-(4-Methyl benzylidene camphor) (4-MBC) was used throughout the study as internal standard. 3-Benzylidene camphor was detected in all tissues at all exposure levels at concentrations between 0.05 μg g-1 (liver) and 36 μg g-1 (adipose tissue) and in plasma at 16-89 μg l-1. The method allowed for the quantification of 3-benzylidene camphor in all tested tissues following topical application. Furthermore, it was shown that 3-benzylidene camphor can be found in various tissues in the rat following topical application. These findings may suggest that following use of 3-benzylidene camphor containing sunscreen, similar disposition and distribution may occur in humans.
Original language | English |
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Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
Volume | 834 |
Issue number | 1-2 |
Pages (from-to) | 117-121 |
ISSN | 1570-0232 |
DOIs | |
Publication status | Published - 2006 |
Externally published | Yes |
- Adipose tissue, Brain, Estrogenic effect, In vivo, Liver, Mass spectrometry, Muscle, Plasma, Sunscreen, Testis
Research areas
ID: 324129035