Distribution of the UV filter 3-benzylidene camphor in rat following topical application
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Distribution of the UV filter 3-benzylidene camphor in rat following topical application. / Søeborg, Tue; Ganderup, Niels Christian; Kristensen, Jakob Højer; Bjerregaard, Poul; Pedersen, Knud Ladegaard; Bollen, Peter; Hansen, Steen Honoré; Halling-Sørensen, Bent.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 834, No. 1-2, 2006, p. 117-121.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Distribution of the UV filter 3-benzylidene camphor in rat following topical application
AU - Søeborg, Tue
AU - Ganderup, Niels Christian
AU - Kristensen, Jakob Højer
AU - Bjerregaard, Poul
AU - Pedersen, Knud Ladegaard
AU - Bollen, Peter
AU - Hansen, Steen Honoré
AU - Halling-Sørensen, Bent
PY - 2006
Y1 - 2006
N2 - A straightforward analytical method for determination of 3-benzylidene camphor (3-BC) in rat adipose tissue, brain, liver, muscle, plasma and testis following topical application was developed and validated. Three exposure levels (60, 180 and 540 mg kg-1 day-1) were tested for 65 days in male Sprague-Dawley rats (24 days postnatal). Sample preparation involving homogenization and n-heptane or methanol extraction of the tissue was applied before injection into the LC-ESI-MS-MS system. The response was linear from 2 to 100 μg l-1 for the qualifier and the quantifier MRM transitions (R2 (quantifier) > 0.994). Detection limit of the method corresponded to 0.005 μg g-1 tissue and 12.5 μg l-1 plasma, respectively. Recovery was determined for all tissues (adipose tissue: 40%; all other tissues: 80-100%) at three individual levels. 3-(4-Methyl benzylidene camphor) (4-MBC) was used throughout the study as internal standard. 3-Benzylidene camphor was detected in all tissues at all exposure levels at concentrations between 0.05 μg g-1 (liver) and 36 μg g-1 (adipose tissue) and in plasma at 16-89 μg l-1. The method allowed for the quantification of 3-benzylidene camphor in all tested tissues following topical application. Furthermore, it was shown that 3-benzylidene camphor can be found in various tissues in the rat following topical application. These findings may suggest that following use of 3-benzylidene camphor containing sunscreen, similar disposition and distribution may occur in humans.
AB - A straightforward analytical method for determination of 3-benzylidene camphor (3-BC) in rat adipose tissue, brain, liver, muscle, plasma and testis following topical application was developed and validated. Three exposure levels (60, 180 and 540 mg kg-1 day-1) were tested for 65 days in male Sprague-Dawley rats (24 days postnatal). Sample preparation involving homogenization and n-heptane or methanol extraction of the tissue was applied before injection into the LC-ESI-MS-MS system. The response was linear from 2 to 100 μg l-1 for the qualifier and the quantifier MRM transitions (R2 (quantifier) > 0.994). Detection limit of the method corresponded to 0.005 μg g-1 tissue and 12.5 μg l-1 plasma, respectively. Recovery was determined for all tissues (adipose tissue: 40%; all other tissues: 80-100%) at three individual levels. 3-(4-Methyl benzylidene camphor) (4-MBC) was used throughout the study as internal standard. 3-Benzylidene camphor was detected in all tissues at all exposure levels at concentrations between 0.05 μg g-1 (liver) and 36 μg g-1 (adipose tissue) and in plasma at 16-89 μg l-1. The method allowed for the quantification of 3-benzylidene camphor in all tested tissues following topical application. Furthermore, it was shown that 3-benzylidene camphor can be found in various tissues in the rat following topical application. These findings may suggest that following use of 3-benzylidene camphor containing sunscreen, similar disposition and distribution may occur in humans.
KW - Adipose tissue
KW - Brain
KW - Estrogenic effect
KW - In vivo
KW - Liver
KW - Mass spectrometry
KW - Muscle
KW - Plasma
KW - Sunscreen
KW - Testis
U2 - 10.1016/j.jchromb.2006.02.026
DO - 10.1016/j.jchromb.2006.02.026
M3 - Journal article
C2 - 16517225
AN - SCOPUS:33646002684
VL - 834
SP - 117
EP - 121
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1-2
ER -
ID: 324129035