Distinct antigenic characteristics of murine parietal yolk sac laminin

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Distinct antigenic characteristics of murine parietal yolk sac laminin. / Wewer, U M; Tichy, D; Damjanov, A; Paulsson, M; Damjanov, I.

In: Developmental Biology, Vol. 121, No. 2, 01.06.1987, p. 397-407.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wewer, UM, Tichy, D, Damjanov, A, Paulsson, M & Damjanov, I 1987, 'Distinct antigenic characteristics of murine parietal yolk sac laminin', Developmental Biology, vol. 121, no. 2, pp. 397-407.

APA

Wewer, U. M., Tichy, D., Damjanov, A., Paulsson, M., & Damjanov, I. (1987). Distinct antigenic characteristics of murine parietal yolk sac laminin. Developmental Biology, 121(2), 397-407.

Vancouver

Wewer UM, Tichy D, Damjanov A, Paulsson M, Damjanov I. Distinct antigenic characteristics of murine parietal yolk sac laminin. Developmental Biology. 1987 Jun 1;121(2):397-407.

Author

Wewer, U M ; Tichy, D ; Damjanov, A ; Paulsson, M ; Damjanov, I. / Distinct antigenic characteristics of murine parietal yolk sac laminin. In: Developmental Biology. 1987 ; Vol. 121, No. 2. pp. 397-407.

Bibtex

@article{c5832d3070624371919762c408d0a057,
title = "Distinct antigenic characteristics of murine parietal yolk sac laminin",
abstract = "Two monoclonal antibodies (LAM-A and LAM-B) specific for laminin from normal and neoplastic parietal yolk sac (PYS) cells were produced in rats immunized with a mouse yolk sac carcinoma cell line. Both antibodies immunoprecipitated the 400,000- and 200,000-Da chains of laminin and reacted with purified PYS laminin in ELISA. LAM-A reacted with mouse and rat PYS laminin, whereas LAM-B reacted only with mouse PYS laminin. Formaldehyde- and methanol-fixed adult and fetal somatic tissues were immunohistochemically unreactive with either of the two antibodies. In acetone-fixed tissue sections, both antibodies reacted weakly with some medullary tubules of the kidney, the follicular basement membrane of the ovaries, and the seminiferous tubules. The antibodies appear to react with the polypeptide determinants residing on the terminal portion of the long arm of laminin. It is concluded that laminin derived from normal or malignant PYS cells has distinct antigenic sites that are immunochemically not apparent in most other basement membranes.",
keywords = "Animals, Antibodies, Monoclonal, Antibody Specificity, Basement Membrane, Blastocyst, Cell Line, Epitopes, Female, Histocytochemistry, Immunologic Tests, Kidney, Laminin, Male, Mesonephroma, Mice, Ovary, Rats, Seminiferous Tubules, Yolk Sac",
author = "Wewer, {U M} and D Tichy and A Damjanov and M Paulsson and I Damjanov",
year = "1987",
month = jun,
day = "1",
language = "English",
volume = "121",
pages = "397--407",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Distinct antigenic characteristics of murine parietal yolk sac laminin

AU - Wewer, U M

AU - Tichy, D

AU - Damjanov, A

AU - Paulsson, M

AU - Damjanov, I

PY - 1987/6/1

Y1 - 1987/6/1

N2 - Two monoclonal antibodies (LAM-A and LAM-B) specific for laminin from normal and neoplastic parietal yolk sac (PYS) cells were produced in rats immunized with a mouse yolk sac carcinoma cell line. Both antibodies immunoprecipitated the 400,000- and 200,000-Da chains of laminin and reacted with purified PYS laminin in ELISA. LAM-A reacted with mouse and rat PYS laminin, whereas LAM-B reacted only with mouse PYS laminin. Formaldehyde- and methanol-fixed adult and fetal somatic tissues were immunohistochemically unreactive with either of the two antibodies. In acetone-fixed tissue sections, both antibodies reacted weakly with some medullary tubules of the kidney, the follicular basement membrane of the ovaries, and the seminiferous tubules. The antibodies appear to react with the polypeptide determinants residing on the terminal portion of the long arm of laminin. It is concluded that laminin derived from normal or malignant PYS cells has distinct antigenic sites that are immunochemically not apparent in most other basement membranes.

AB - Two monoclonal antibodies (LAM-A and LAM-B) specific for laminin from normal and neoplastic parietal yolk sac (PYS) cells were produced in rats immunized with a mouse yolk sac carcinoma cell line. Both antibodies immunoprecipitated the 400,000- and 200,000-Da chains of laminin and reacted with purified PYS laminin in ELISA. LAM-A reacted with mouse and rat PYS laminin, whereas LAM-B reacted only with mouse PYS laminin. Formaldehyde- and methanol-fixed adult and fetal somatic tissues were immunohistochemically unreactive with either of the two antibodies. In acetone-fixed tissue sections, both antibodies reacted weakly with some medullary tubules of the kidney, the follicular basement membrane of the ovaries, and the seminiferous tubules. The antibodies appear to react with the polypeptide determinants residing on the terminal portion of the long arm of laminin. It is concluded that laminin derived from normal or malignant PYS cells has distinct antigenic sites that are immunochemically not apparent in most other basement membranes.

KW - Animals

KW - Antibodies, Monoclonal

KW - Antibody Specificity

KW - Basement Membrane

KW - Blastocyst

KW - Cell Line

KW - Epitopes

KW - Female

KW - Histocytochemistry

KW - Immunologic Tests

KW - Kidney

KW - Laminin

KW - Male

KW - Mesonephroma

KW - Mice

KW - Ovary

KW - Rats

KW - Seminiferous Tubules

KW - Yolk Sac

M3 - Journal article

C2 - 2438172

VL - 121

SP - 397

EP - 407

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -

ID: 34330273