Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark
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Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark. / Samuelsen, Ørjan; Hansen, Frank; Aasnæs, Bettina; Hasman, Henrik; Lund, Bjarte Aarmo; Leiros, Hanna Kirsti S.; Lilje, Berit; Janice, Jessin; Jakobsen, Lotte; Littauer, Pia; Søes, Lillian M.; Holzknecht, Barbara J.; Andersen, Leif P.; Stegger, Marc; Andersen, Paal S.; Hammerum, Anette M.
In: Antimicrobial Agents and Chemotherapy, Vol. 62, No. 1, e01260-17, 01.2018.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dissemination and characteristics of a novel plasmid-encoded carbapenem-hydrolyzing class D β-lactamase, OXA-436, found in isolates from four patients at six different hospitals in Denmark
AU - Samuelsen, Ørjan
AU - Hansen, Frank
AU - Aasnæs, Bettina
AU - Hasman, Henrik
AU - Lund, Bjarte Aarmo
AU - Leiros, Hanna Kirsti S.
AU - Lilje, Berit
AU - Janice, Jessin
AU - Jakobsen, Lotte
AU - Littauer, Pia
AU - Søes, Lillian M.
AU - Holzknecht, Barbara J.
AU - Andersen, Leif P.
AU - Stegger, Marc
AU - Andersen, Paal S.
AU - Hammerum, Anette M.
PY - 2018/1
Y1 - 2018/1
N2 - The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D β-lactamase (CHDL) named OXA-436. In total, six OXA-436-producing Enterobacteriaceae isolates, including Enterobacter asburiae (n = 3), Citrobacter freundii (n = 2), and Klebsiella pneumoniae (n = 1), were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing Enterobacteriaceae were found in one patient. The amino acid sequence of OXA-436 showed 90.4 to 92.8% identity to the amino acid sequences of other acquired OXA-48-like variants. Expression of OXA-436 in Escherichia coli and kinetic analysis of purified OXA-436 revealed an activity profile similar to that of OXA-48 and OXA-181, with activity against penicillins, including temocillin; limited or no activity against extended-spectrum cephalosporins; and activity against carbapenems. The blaOXA-436 gene was located on a conjugative ∼314-kb IncHI2/IncHI2A plasmid belonging to plasmid multilocus sequence typing sequence type 1 in a region surrounded by chromosomal genes previously identified to be adjacent to blaOXA genes in Shewanella spp. In conclusion, OXA-436 is a novel CHDL with functional properties similar to those of OXA-48-like CHDLs. The described geographical spread among different Enterobacteriaceae and the plasmid location of blaOXA-436 illustrate its potential for further dissemination.
AB - The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D β-lactamase (CHDL) named OXA-436. In total, six OXA-436-producing Enterobacteriaceae isolates, including Enterobacter asburiae (n = 3), Citrobacter freundii (n = 2), and Klebsiella pneumoniae (n = 1), were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing Enterobacteriaceae were found in one patient. The amino acid sequence of OXA-436 showed 90.4 to 92.8% identity to the amino acid sequences of other acquired OXA-48-like variants. Expression of OXA-436 in Escherichia coli and kinetic analysis of purified OXA-436 revealed an activity profile similar to that of OXA-48 and OXA-181, with activity against penicillins, including temocillin; limited or no activity against extended-spectrum cephalosporins; and activity against carbapenems. The blaOXA-436 gene was located on a conjugative ∼314-kb IncHI2/IncHI2A plasmid belonging to plasmid multilocus sequence typing sequence type 1 in a region surrounded by chromosomal genes previously identified to be adjacent to blaOXA genes in Shewanella spp. In conclusion, OXA-436 is a novel CHDL with functional properties similar to those of OXA-48-like CHDLs. The described geographical spread among different Enterobacteriaceae and the plasmid location of blaOXA-436 illustrate its potential for further dissemination.
KW - Carbapenems
U2 - 10.1128/AAC.01260-17
DO - 10.1128/AAC.01260-17
M3 - Journal article
C2 - 29061750
AN - SCOPUS:85039785235
VL - 62
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 1
M1 - e01260-17
ER -
ID: 188752716