Direct inhibition of RNA polymerase II transcription by RECQL5

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Direct inhibition of RNA polymerase II transcription by RECQL5. / Aygün, Ozan; Xu, Xiaohua; Liu, Yilun; Takahashi, Hidehisa; Kong, Stephanie E.; Conaway, Ronald C.; Conaway, Joan W.; Svejstrup, Jesper Q.

In: Journal of Biological Chemistry, Vol. 284, No. 35, 28.08.2009, p. 23197-23203.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Aygün, O, Xu, X, Liu, Y, Takahashi, H, Kong, SE, Conaway, RC, Conaway, JW & Svejstrup, JQ 2009, 'Direct inhibition of RNA polymerase II transcription by RECQL5', Journal of Biological Chemistry, vol. 284, no. 35, pp. 23197-23203. https://doi.org/10.1074/jbc.M109.015750

APA

Aygün, O., Xu, X., Liu, Y., Takahashi, H., Kong, S. E., Conaway, R. C., Conaway, J. W., & Svejstrup, J. Q. (2009). Direct inhibition of RNA polymerase II transcription by RECQL5. Journal of Biological Chemistry, 284(35), 23197-23203. https://doi.org/10.1074/jbc.M109.015750

Vancouver

Aygün O, Xu X, Liu Y, Takahashi H, Kong SE, Conaway RC et al. Direct inhibition of RNA polymerase II transcription by RECQL5. Journal of Biological Chemistry. 2009 Aug 28;284(35):23197-23203. https://doi.org/10.1074/jbc.M109.015750

Author

Aygün, Ozan ; Xu, Xiaohua ; Liu, Yilun ; Takahashi, Hidehisa ; Kong, Stephanie E. ; Conaway, Ronald C. ; Conaway, Joan W. ; Svejstrup, Jesper Q. / Direct inhibition of RNA polymerase II transcription by RECQL5. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 35. pp. 23197-23203.

Bibtex

@article{caf1a716332f421ea1ace66190b087cf,
title = "Direct inhibition of RNA polymerase II transcription by RECQL5",
abstract = "DNA helicases of the RECQ family are important for maintaining genome integrity, from bacteria to humans. Although progress has been made in understanding the biochemical role of some human RECQ helicases, that of RECQL5 remains elusive. We recently reported that RECQL5 interacts with RNA polymerase II (RNAPII), pointing to a role for the protein in transcription. Here, we show that RECQL5 inhibits both initiation and elongation in transcription assays reconstituted with highly purified general transcription factors and RNAPII. Such inhibition is not observed with the related, much more active RECQL1 helicase or with a version of RECQL5 that has normal helicase activity but is impaired in its ability to interact with RNAPII. Indeed, RECQL5 helicase activity is not required for inhibition. We discuss our findings in light of the fact RECQ5-/- mice have elevated levels of DNA recombination and a higher incidence of cancer.",
author = "Ozan Ayg{\"u}n and Xiaohua Xu and Yilun Liu and Hidehisa Takahashi and Kong, {Stephanie E.} and Conaway, {Ronald C.} and Conaway, {Joan W.} and Svejstrup, {Jesper Q.}",
year = "2009",
month = aug,
day = "28",
doi = "10.1074/jbc.M109.015750",
language = "English",
volume = "284",
pages = "23197--23203",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "35",

}

RIS

TY - JOUR

T1 - Direct inhibition of RNA polymerase II transcription by RECQL5

AU - Aygün, Ozan

AU - Xu, Xiaohua

AU - Liu, Yilun

AU - Takahashi, Hidehisa

AU - Kong, Stephanie E.

AU - Conaway, Ronald C.

AU - Conaway, Joan W.

AU - Svejstrup, Jesper Q.

PY - 2009/8/28

Y1 - 2009/8/28

N2 - DNA helicases of the RECQ family are important for maintaining genome integrity, from bacteria to humans. Although progress has been made in understanding the biochemical role of some human RECQ helicases, that of RECQL5 remains elusive. We recently reported that RECQL5 interacts with RNA polymerase II (RNAPII), pointing to a role for the protein in transcription. Here, we show that RECQL5 inhibits both initiation and elongation in transcription assays reconstituted with highly purified general transcription factors and RNAPII. Such inhibition is not observed with the related, much more active RECQL1 helicase or with a version of RECQL5 that has normal helicase activity but is impaired in its ability to interact with RNAPII. Indeed, RECQL5 helicase activity is not required for inhibition. We discuss our findings in light of the fact RECQ5-/- mice have elevated levels of DNA recombination and a higher incidence of cancer.

AB - DNA helicases of the RECQ family are important for maintaining genome integrity, from bacteria to humans. Although progress has been made in understanding the biochemical role of some human RECQ helicases, that of RECQL5 remains elusive. We recently reported that RECQL5 interacts with RNA polymerase II (RNAPII), pointing to a role for the protein in transcription. Here, we show that RECQL5 inhibits both initiation and elongation in transcription assays reconstituted with highly purified general transcription factors and RNAPII. Such inhibition is not observed with the related, much more active RECQL1 helicase or with a version of RECQL5 that has normal helicase activity but is impaired in its ability to interact with RNAPII. Indeed, RECQL5 helicase activity is not required for inhibition. We discuss our findings in light of the fact RECQ5-/- mice have elevated levels of DNA recombination and a higher incidence of cancer.

U2 - 10.1074/jbc.M109.015750

DO - 10.1074/jbc.M109.015750

M3 - Journal article

C2 - 19570979

AN - SCOPUS:69949145039

VL - 284

SP - 23197

EP - 23203

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 35

ER -

ID: 330999947