Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors
Research output: Contribution to journal › Journal article › Research › peer-review
Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.
Original language | English |
---|---|
Journal | Current Protocols in Stem Cell Biology |
Volume | 36 |
Pages (from-to) | 1G.3.1-12 |
ISSN | 1938-8969 |
DOIs | |
Publication status | Published - 3 Feb 2016 |
- Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 167471162